Hotspot

petA-psbJ

LSC · 61259-62256 · 998 bp

Back to Cyclocodon

Pi 0.00811

MarkerSeek score 64.60

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 998
variable_sites: 8
pi: 0.00811
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	CTTTTGCTCTTCTTGGCAGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.9	57.6	1111-1123 bp	1.0	78.0
petA-psbJ_p2	GCTCTTCTTGGCAGCTGTTA	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.2	57.6	1106-1118 bp	1.0	77.0
petA-psbJ_p3	TTTTGCTCTTCTTGGCAGCT	ATTCCGGATTGGGTTCATCC	45.0	50.0	58.3	57.6	1110-1122 bp	1.0	76.1
petA-psbJ_p4	CGTGTCCAAGGTCTTTTGCT	ATTCCGGATTGGGTTCATCC	50.0	50.0	58.7	57.6	1123-1135 bp	1.0	73.0
petA-psbJ_p5	GGTCTTTTGCTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	50.0	50.0	57.3	57.6	1114-1126 bp	1.0	72.8