Hotspot

trnC-GCA-trnM-CAU

LSC · 45684-47391 · 1708 bp

Back to Cyclocodon

Pi 0.0462

MarkerSeek score 75.00

Primer available yes

Label trnC-GCA-trnM-CAU

Feature Metadata

label_name: trnC-GCA-trnM-CAU
feature_type: igs
region: LSC
length_bp: 1708
variable_sites: 77
pi: 0.0462
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-trnM-CAU_p1	ATTTGCAGTCCCCCGCCTTA	GGATGGGTCCCATTTGGTTT	55.0	50.0	61.9	58.0	1856-2203 bp	1.0	53.4
trnC-GCA-trnM-CAU_p2	ATTTGCAGTCCCCCGCCTTA	TATTGCTTTCATACGGCGGG	55.0	50.0	61.9	58.4	1795-2142 bp	1.0	50.6
trnC-GCA-trnM-CAU_p3	ATTTGCAGTCCCCCGCCTTA	GTATTGCTTTCATACGGCGG	55.0	50.0	61.9	57.3	1796-2143 bp	1.0	48.6
trnC-GCA-trnM-CAU_p4	TTTGCAGTCCCCCGCCTTA	GGATGGGTCCCATTTGGTTT	57.9	50.0	61.5	58.0	1855-2202 bp	1.0	48.0
trnC-GCA-trnM-CAU_p5	ATTTGCAGTCCCCCGCCTTA	GCGGGAGTCATTGGTTCAAA	55.0	50.0	61.9	58.8	1780-2127 bp	1.0	47.9