Hotspot

trnT-GGU-psbD

LSC · 16349-17452 · 1104 bp

Back to Cyclocodon

Pi 0.0136

MarkerSeek score 66.60

Primer available yes

Label trnT-GGU-psbD

Feature Metadata

label_name: trnT-GGU-psbD
feature_type: igs
region: LSC
length_bp: 1104
variable_sites: 15
pi: 0.0136
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-psbD_p1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	55.0	50.0	58.1	58.1	1264-1335 bp	1.0	79.5
trnT-GGU-psbD_p2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1267-1338 bp	1.0	79.3
trnT-GGU-psbD_p3	AACACGCCCCTTTAACTCAG	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1283-1354 bp	1.0	79.1
trnT-GGU-psbD_p4	GGCGTAAGTCCTTGGTTCAA	GGACCAGCCTACAAAAACGA	50.0	50.0	58.1	58.1	1241-1312 bp	1.0	79.1
trnT-GGU-psbD_p5	GTGGTAGAGTAACGCCATGG	CAGCCTACAAAAACGAACCG	55.0	50.0	58.1	57.7	1260-1331 bp	1.0	77.9