primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rps16_p1	trnK-UUU-rps16	1	AAAGCCGAGTACTCTACCGT	TTCTTGAAAGGGGTGCTCAG	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.445152	4308	4841	677-678	1.000000	78.9
trnK-UUU-rps16_p2	trnK-UUU-rps16	2	AAGCCGAGTACTCTACCGTT	TTCTTGAAAGGGGTGCTCAG	20	20	50.0	50.0	58.2	57.7	20.3	0.0	0.445152	4308	4841	676-677	1.000000	78.9
trnK-UUU-rps16_p3	trnK-UUU-rps16	3	AAAGCCGAGTACTCTACCGT	GGAGTCTAGTTCAGCGTCAC	20	20	50.0	55.0	58.2	57.7	20.3	0.0	0.434067	4308	4841	1177-1178	1.000000	78.4
trnK-UUU-rps16_p4	trnK-UUU-rps16	4	AAGCCGAGTACTCTACCGTT	GGAGTCTAGTTCAGCGTCAC	20	20	50.0	55.0	58.2	57.7	20.3	0.0	0.434067	4308	4841	1176-1177	1.000000	78.4
trnK-UUU-rps16_p5	trnK-UUU-rps16	5	TCAGTCGTGGTCTTCCAAAC	GGAGTCTAGTTCAGCGTCAC	20	20	50.0	55.0	57.8	57.7	0.0	0.0	0.499947	4308	4841	1262-1263	1.000000	77.8
rps16-trnQ-UUG_p1	rps16-trnQ-UUG	1	TGACTTGTGTTGGATTGGCA	GGGTTTTGATCCCGGCATAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.069057	5118	7070	2410-2410	0.500000	59.4
rps16-trnQ-UUG_p2	rps16-trnQ-UUG	2	ACTTGTGTTGGATTGGCACT	GGGTTTTGATCCCGGCATAT	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.076401	5118	7070	2408-2408	0.500000	59.4
rps16-trnQ-UUG_p3	rps16-trnQ-UUG	3	TGACTTGTGTTGGATTGGCA	AGATATTGGCGAGCTGTTGG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.105392	5118	7070	2612-2612	0.500000	59.2
rps16-trnQ-UUG_p4	rps16-trnQ-UUG	4	ACTTGTGTTGGATTGGCACT	AGATATTGGCGAGCTGTTGG	20	20	45.0	50.0	57.9	58.0	0.0	0.0	0.112736	5118	7070	2610-2610	0.500000	59.1
rps16-trnQ-UUG_p5	rps16-trnQ-UUG	5	TGACTTGTGTTGGATTGGCA	TTGGCAAACGGACGTTTTTC	20	20	45.0	45.0	57.9	58.1	0.0	18.9	0.153746	5118	7070	2596-2596	0.500000	58.8
trnQ-UUG_p1	trnQ-UUG	1	TTAGTCCAACCAAGCCAACC	CGAAAACTTACAGCGGCTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059822	7071	7142	1351-1354	1.000000	82.7
trnQ-UUG_p2	trnQ-UUG	2	TTAGTCCAACCAAGCCAACC	ATCGAAAACTTACAGCGGCT	20	20	50.0	45.0	58.0	57.9	0.0	0.0	0.105056	7071	7142	1353-1356	1.000000	82.3
trnQ-UUG_p3	trnQ-UUG	3	GTCCAACCAAGCCAACCATA	CGAAAACTTACAGCGGCTTG	20	20	50.0	50.0	58.1	58.0	0.0	0.0	0.131656	7071	7142	1348-1351	1.000000	82.1
trnQ-UUG_p4	trnQ-UUG	4	TTAGTCCAACCAAGCCAACC	TCGAAAACTTACAGCGGCTT	20	20	50.0	45.0	58.0	58.1	0.0	0.0	0.136596	7071	7142	1352-1355	1.000000	82.1
trnQ-UUG_p5	trnQ-UUG	5	TTAGTCCAACCAAGCCAACC	GGGCAATTTGGCGAAGAAAA	20	20	50.0	45.0	58.0	57.8	0.0	15.8	0.172613	7071	7142	1250-1253	1.000000	82.1
trnQ-UUG-psbK_p1	trnQ-UUG-psbK	1	ATATGCCGGGATCAAAACCC	CGAAAACTTACAGCGGCTTG	20	20	50.0	50.0	58.0	58.0	13.5	0.0	0.054152	7143	7692	776-779	1.000000	83.4
trnQ-UUG-psbK_p2	trnQ-UUG-psbK	2	TTAGTCCAACCAAGCCAACC	CGAAAACTTACAGCGGCTTG	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.059822	7143	7692	1351-1354	1.000000	82.7
trnQ-UUG-psbK_p3	trnQ-UUG-psbK	3	ATATGCCGGGATCAAAACCC	CTTCACGTCCAGGATTACGG	20	20	50.0	55.0	58.0	58.1	13.5	4.3	0.082053	7143	7692	1336-1339	1.000000	82.0
trnQ-UUG-psbK_p4	trnQ-UUG-psbK	4	ATATGCCGGGATCAAAACCC	AAAAGGAAAAGGGCCGGAAT	20	20	50.0	45.0	58.0	58.0	13.5	5.0	0.028799	7143	7692	958-958	0.500000	59.8
trnQ-UUG-psbK_p5	trnQ-UUG-psbK	5	TTAGTCCAACCAAGCCAACC	AAAAGGAAAAGGGCCGGAAT	20	20	50.0	45.0	58.0	58.0	0.0	5.0	0.034469	7143	7692	1533-1533	0.500000	59.7
atpH-trnD-GUC_p1	atpH-trnD-GUC	1	ATAACGGAAGCGGCAGAAAT	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	58.0	58.5	1.4	5.4	0.515719	14055	15090	1131-1132	1.000000	76.9
atpH-trnD-GUC_p2	atpH-trnD-GUC	2	AGCCAATCCAGCAGCAATAA	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	57.9	58.5	0.0	5.4	0.625777	14055	15090	1147-1148	1.000000	76.0
atpH-trnD-GUC_p3	atpH-trnD-GUC	3	AATAACGGAAGCGGCAGAAA	TTCAATTGGTCAGAGCACCG	20	20	45.0	50.0	58.2	58.5	1.4	5.4	0.661257	14055	15090	1132-1133	1.000000	75.7
atpH-trnD-GUC_p4	atpH-trnD-GUC	4	GCCAATCCAGCAGCAATAAC	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	57.8	58.5	0.0	5.4	0.691719	14055	15090	1146-1147	1.000000	75.4
atpH-trnD-GUC_p5	atpH-trnD-GUC	5	TCCAGCAGCAATAACGGAAG	TTCAATTGGTCAGAGCACCG	20	20	50.0	50.0	58.3	58.5	0.0	5.4	0.739493	14055	15090	1141-1142	1.000000	75.1
trnY-GUA-trnE-UUC_p1	trnY-GUA-trnE-UUC	1	CCAGCGTAGACATATTGCCA	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.336052	15358	15416	202-202	1.000000	78.1
trnY-GUA-trnE-UUC_p2	trnY-GUA-trnE-UUC	2	ACCAGCGTAGACATATTGCC	AAGTATGCCCCCATCGTCTA	20	20	50.0	50.0	57.8	57.9	0.0	0.0	0.337603	15358	15416	203-203	1.000000	78.1
trnY-GUA-trnE-UUC_p3	trnY-GUA-trnE-UUC	3	CCAGCGTAGACATATTGCCA	TCTCTTTCAAGGAGGCAACG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.408774	15358	15416	167-167	1.000000	77.7
trnY-GUA-trnE-UUC_p4	trnY-GUA-trnE-UUC	4	ACCAGCGTAGACATATTGCC	TCTCTTTCAAGGAGGCAACG	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.410326	15358	15416	168-168	1.000000	77.7
trnY-GUA-trnE-UUC_p5	trnY-GUA-trnE-UUC	5	CGAATTTACAGTCCGTCCCC	AAGTATGCCCCCATCGTCTA	20	20	55.0	50.0	58.4	57.9	0.0	0.0	0.448606	15358	15416	181-181	1.000000	77.3
trnT-GGU-psbD_p1	trnT-GGU-psbD	1	GTGGTAGAGTAACGCCATGG	GGACCAGCCTACAAAAACGA	20	20	55.0	50.0	58.1	58.1	7.6	0.0	0.177181	16349	17452	1264-1335	1.000000	79.5
trnT-GGU-psbD_p2	trnT-GGU-psbD	2	TCAGTGGTAGAGTAACGCCA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.199336	16349	17452	1267-1338	1.000000	79.3
trnT-GGU-psbD_p3	trnT-GGU-psbD	3	AACACGCCCCTTTAACTCAG	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.220904	16349	17452	1283-1354	1.000000	79.1
trnT-GGU-psbD_p4	trnT-GGU-psbD	4	GGCGTAAGTCCTTGGTTCAA	GGACCAGCCTACAAAAACGA	20	20	50.0	50.0	58.1	58.1	0.0	0.0	0.226227	16349	17452	1241-1312	1.000000	79.1
trnT-GGU-psbD_p5	trnT-GGU-psbD	5	GTGGTAGAGTAACGCCATGG	CAGCCTACAAAAACGAACCG	20	20	55.0	50.0	58.1	57.7	7.6	0.0	0.373965	16349	17452	1260-1331	1.000000	77.9
trnG-GCC-trnfM-CAU_p1	trnG-GCC-trnfM-CAU	1	ATGCGGATATGGTCGAATGG	CTTGAGGTCACCGGTTCAAA	20	20	50.0	50.0	57.9	58.0	0.0	17.7	0.137348	21051	21225	282-282	1.000000	80.9
trnG-GCC-trnfM-CAU_p2	trnG-GCC-trnfM-CAU	2	ATGCGGATATGGTCGAATGG	AGTTCTTCTCTTAAGCGCGG	20	20	50.0	50.0	57.9	58.0	0.0	8.8	0.101472	21051	21225	337-337	1.000000	80.8
trnG-GCC-trnfM-CAU_p3	trnG-GCC-trnfM-CAU	3	ATGCGGATATGGTCGAATGG	GTTCTTCTCTTAAGCGCGGA	20	20	50.0	50.0	57.9	58.0	0.0	8.8	0.106972	21051	21225	336-336	1.000000	80.8
trnG-GCC-trnfM-CAU_p4	trnG-GCC-trnfM-CAU	4	ATGCGGATATGGTCGAATGG	TCTCTTAAGCGCGGAGTAGA	20	20	50.0	50.0	57.9	58.0	0.0	8.8	0.133246	21051	21225	331-331	1.000000	80.6
trnG-GCC-trnfM-CAU_p5	trnG-GCC-trnfM-CAU	5	TCTTTGCCAAGGAGAAGACG	AGTTCTTCTCTTAAGCGCGG	20	20	50.0	50.0	57.8	58.0	0.0	8.8	0.172163	21051	21225	308-308	1.000000	80.4
rpoB-rpoC1_p1	rpoB-rpoC1	1	ACGATCTTTGGCTCTGGAAC	TTTGTCACCTCGCCAACTAG	20	20	50.0	50.0	57.9	57.8	0.0	0.0	0.264423	34831	34859	221-221	1.000000	78.2
rpoB-rpoC1_p2	rpoB-rpoC1	2	ACGATCTTTGGCTCTGGAAC	AGGCAGGATTTTATTGGCCC	20	20	50.0	50.0	57.9	58.2	0.0	0.0	0.307191	34831	34859	192-192	1.000000	78.0
rpoB-rpoC1_p3	rpoB-rpoC1	3	CGACTGCTTGTTCGAGAACT	TTTGTCACCTCGCCAACTAG	20	20	50.0	50.0	58.2	57.8	15.8	0.0	0.391635	34831	34859	241-241	1.000000	77.2
rpoB-rpoC1_p4	rpoB-rpoC1	4	TCGACTGCTTGTTCGAGAAC	TTTGTCACCTCGCCAACTAG	20	20	50.0	50.0	58.2	57.8	8.9	0.0	0.396529	34831	34859	242-242	1.000000	77.2
rpoB-rpoC1_p5	rpoB-rpoC1	5	CGACTGCTTGTTCGAGAACT	AGGCAGGATTTTATTGGCCC	20	20	50.0	50.0	58.2	58.2	15.8	0.0	0.434404	34831	34859	212-212	1.000000	76.9
trnC-GCA-trnM-CAU_p1	trnC-GCA-trnM-CAU	1	ATTTGCAGTCCCCCGCCTTA	GGATGGGTCCCATTTGGTTT	20	20	55.0	50.0	61.9	58.0	0.0	24.8	3.907529	45684	47391	1856-2203	1.000000	53.4
trnC-GCA-trnM-CAU_p2	trnC-GCA-trnM-CAU	2	ATTTGCAGTCCCCCGCCTTA	TATTGCTTTCATACGGCGGG	20	20	55.0	50.0	61.9	58.4	0.0	13.2	4.270065	45684	47391	1795-2142	1.000000	50.6
trnC-GCA-trnM-CAU_p3	trnC-GCA-trnM-CAU	3	ATTTGCAGTCCCCCGCCTTA	GTATTGCTTTCATACGGCGG	20	20	55.0	50.0	61.9	57.3	0.0	13.2	4.522018	45684	47391	1796-2143	1.000000	48.6
trnC-GCA-trnM-CAU_p4	trnC-GCA-trnM-CAU	4	TTTGCAGTCCCCCGCCTTA	GGATGGGTCCCATTTGGTTT	19	20	57.9	50.0	61.5	58.0	0.0	24.8	4.579463	45684	47391	1855-2202	1.000000	48.0
trnC-GCA-trnM-CAU_p5	trnC-GCA-trnM-CAU	5	ATTTGCAGTCCCCCGCCTTA	GCGGGAGTCATTGGTTCAAA	20	20	55.0	50.0	61.9	58.8	0.0	0.0	4.614837	45684	47391	1780-2127	1.000000	47.9
trnV-UAC-ndhC_p1	trnV-UAC-ndhC	1	GGTTCGAATCCGTATAGCCC	CTGGTCCTTTGCTAATGGGG	20	20	55.0	55.0	57.9	58.2	0.0	0.0	0.308220	48315	49180	1003-1003	1.000000	78.3
trnV-UAC-ndhC_p2	trnV-UAC-ndhC	2	GGTTCGAATCCGTATAGCCC	TCCTTTGCTAATGGGGGCTA	20	20	55.0	50.0	57.9	58.4	0.0	0.0	0.473539	48315	49180	999-999	1.000000	76.9
trnV-UAC-ndhC_p3	trnV-UAC-ndhC	3	GGTTCGAATCCGTATAGCCC	CCTTTGCTAATGGGGGCTAA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.528579	48315	49180	998-998	1.000000	76.5
trnV-UAC-ndhC_p4	trnV-UAC-ndhC	4	CGGTTCGAATCCGTATAGCC	CTGGTCCTTTGCTAATGGGG	20	20	55.0	55.0	58.3	58.2	22.8	0.0	0.537602	48315	49180	1004-1004	1.000000	76.4
trnV-UAC-ndhC_p5	trnV-UAC-ndhC	5	GGTTCGAATCCGTATAGCCC	GGGCTAAAACTCCCGAAAGA	20	20	55.0	50.0	57.9	57.5	0.0	0.0	0.560721	48315	49180	985-985	1.000000	76.2
petA-psbJ_p1	petA-psbJ	1	CTTTTGCTCTTCTTGGCAGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.9	57.6	0.0	4.1	0.426712	61259	62256	1111-1123	1.000000	78.0
petA-psbJ_p2	petA-psbJ	2	GCTCTTCTTGGCAGCTGTTA	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.2	57.6	16.6	4.1	0.551459	61259	62256	1106-1118	1.000000	77.0
petA-psbJ_p3	petA-psbJ	3	TTTTGCTCTTCTTGGCAGCT	ATTCCGGATTGGGTTCATCC	20	20	45.0	50.0	58.3	57.6	0.0	4.1	0.668909	61259	62256	1110-1122	1.000000	76.1
petA-psbJ_p4	petA-psbJ	4	CGTGTCCAAGGTCTTTTGCT	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	58.7	57.6	0.0	4.1	1.055974	61259	62256	1123-1135	1.000000	73.0
petA-psbJ_p5	petA-psbJ	5	GGTCTTTTGCTCTTCTTGGC	ATTCCGGATTGGGTTCATCC	20	20	50.0	50.0	57.3	57.6	0.0	4.1	1.078868	61259	62256	1114-1126	1.000000	72.8
rps12-trnQ-UUG_p1	rps12-trnQ-UUG	1	TGACCCTGTTAGTCCGTTCT	GGGTTTTGATCCCGGCATAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.009994	68477	70904	2897-2897	1.000000	81.1
rps12-trnQ-UUG_p2	rps12-trnQ-UUG	2	CTGAACGAGTCGCACATACA	GGGTTTTGATCCCGGCATAT	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.027435	68477	70904	2602-2602	1.000000	81.0
rps12-trnQ-UUG_p3	rps12-trnQ-UUG	3	TTATGAGGGTTGGCTGGGTA	GGGTTTTGATCCCGGCATAT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.030596	68477	70904	2919-2919	1.000000	80.9
rps12-trnQ-UUG_p4	rps12-trnQ-UUG	4	CTGAACGAGTCGCACATACA	AGATATTGGCGAGCTGTTGG	20	20	50.0	50.0	58.0	58.0	13.6	0.0	0.063770	68477	70904	2804-2804	0.500000	59.5
rps12-trnQ-UUG_p5	rps12-trnQ-UUG	5	AGCGGGGGATTTTGAAACAT	GGGTTTTGATCCCGGCATAT	20	20	45.0	50.0	58.1	58.0	0.0	0.0	0.067433	68477	70904	2542-2542	0.500000	59.5
trnQ-UUG_p1	trnQ-UUG	1	TTAGTCCAACCAAGCCAACC	CATATTCGGCGACCTGTTGA	20	20	50.0	50.0	58.0	58.1	0.0	10.2	0.075705	70905	70976	1094-1097	1.000000	83.4
trnQ-UUG_p2	trnQ-UUG	2	TTAGTCCAACCAAGCCAACC	TCATATTCGGCGACCTGTTG	20	20	50.0	50.0	58.0	58.1	0.0	10.2	0.075705	70905	70976	1095-1098	1.000000	83.4
trnQ-UUG_p3	trnQ-UUG	3	TTAGTCCAACCAAGCCAACC	TACGCAATGGGAAGGTTGAG	20	20	50.0	50.0	58.0	57.9	0.0	3.8	0.118244	70905	70976	1138-1141	1.000000	82.9
trnQ-UUG_p4	trnQ-UUG	4	TTAGTCCAACCAAGCCAACC	ACCTCTTGACACCTAGGCAT	20	20	50.0	50.0	58.0	58.0	0.0	9.3	0.052005	70905	70976	1031-1031	0.500000	59.6
trnQ-UUG_p5	trnQ-UUG	5	GTCCAACCAAGCCAACCATA	ACCTCTTGACACCTAGGCAT	20	20	50.0	50.0	58.1	58.0	0.0	9.3	0.123840	70905	70976	1028-1028	0.500000	59.0
rps3_p1	rps3	1	ATAGAAGGGAAGGGCAAGGT	CCACAAGATGACTATGCATTCG	20	22	50.0	45.5	58.0	57.5	0.0	3.5	2.476436	82030	82686	739-739	1.000000	62.1
rps3_p2	rps3	2	TCGAATAGAAGGGAAGGGCA	CCACAAGATGACTATGCATTCG	20	22	50.0	45.5	58.1	57.5	0.0	3.5	2.589386	82030	82686	743-743	1.000000	61.2
rps3_p3	rps3	3	TAGAAGGGAAGGGCAAGGTT	CCACAAGATGACTATGCATTCG	20	22	50.0	45.5	58.2	57.5	0.0	3.5	2.708315	82030	82686	738-738	1.000000	60.3
rps3_p4	rps3	4	GAAGGGAAGGGCAAGGTTTA	CCACAAGATGACTATGCATTCG	20	22	50.0	45.5	57.4	57.5	0.0	3.5	3.061093	82030	82686	736-736	1.000000	57.5
rps3_p5	rps3	5	ATAGAAGGGAAGGGCAAGGT	CACAAGATGACTATGCATTCGT	20	22	50.0	40.9	58.0	56.9	0.0	3.5	3.144176	82030	82686	738-738	1.000000	56.8
trnL-UAG-rpl32_p1	trnL-UAG-rpl32	1	AGCGTGTCTACCGATTTCAC	TCTAGGTCGGCGTTAAAAGC	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.008260	124072	124920	968-968	1.000000	81.8
trnL-UAG-rpl32_p2	trnL-UAG-rpl32	2	CAGCGTGTCTACCGATTTCA	TCTAGGTCGGCGTTAAAAGC	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.009630	124072	124920	969-969	1.000000	81.8
trnL-UAG-rpl32_p3	trnL-UAG-rpl32	3	AGCGTGTCTACCGATTTCAC	CTAGGTCGGCGTTAAAAGCT	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.013801	124072	124920	967-967	1.000000	81.8
trnL-UAG-rpl32_p4	trnL-UAG-rpl32	4	CAGCGTGTCTACCGATTTCA	CTAGGTCGGCGTTAAAAGCT	20	20	50.0	50.0	58.0	58.0	0.0	6.8	0.015171	124072	124920	968-968	1.000000	81.8
trnL-UAG-rpl32_p5	trnL-UAG-rpl32	5	AGCGTGTCTACCGATTTCAC	GGCGTTAAAAGCTTTGGCAT	20	20	50.0	45.0	58.0	57.9	0.0	13.5	0.086679	124072	124920	960-960	1.000000	81.2
ycf1_p1	ycf1	1	ACTCCGAGCCCATAGTCTAC	TCTTTCACAGAGTCCCTCGT	20	20	55.0	50.0	58.0	58.0	0.0	0.0	0.019136	138112	138231	1129-1210	1.000000	81.8
ycf1_p2	ycf1	2	ACCACTCGGTCTTTTGCAAT	TCTTTCACAGAGTCCCTCGT	20	20	45.0	50.0	58.0	58.0	0.0	0.0	0.021945	138112	138231	1242-1323	1.000000	81.7
ycf1_p3	ycf1	3	AGTCCCCAGAAAGCCCTTAT	TCTTTCACAGAGTCCCTCGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.016122	138112	138231	1453-1534	1.000000	81.6
ycf1_p4	ycf1	4	ATAGTCCCCAGAAAGCCCTT	TCTTTCACAGAGTCCCTCGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.016122	138112	138231	1455-1536	1.000000	81.6
ycf1_p5	ycf1	5	GTTTATGGGTTTGACGTGCG	TCTTTCACAGAGTCCCTCGT	20	20	50.0	50.0	58.0	58.0	0.0	0.0	0.044785	138112	138231	1040-1040	0.500000	59.6