Hotspot

ndhD

SSC · 121084-122613 · 1530 bp

Back to Cyclea

Pi 0.00458
MarkerSeek score 47.40
Primer available yes
Label ndhD

Feature Metadata

label_name
ndhD
feature_type
gene
region
SSC
length_bp
1530
variable_sites
7
pi
0.00458
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhD_p1 GTCCCTATCCAACAAACGCT TGATACCTTGGGATGGGTGT 50.0 50.0 57.9 58.0 1868 bp 1.0 79.6
ndhD_p2 AATGAGGCATGGGGATCCTA TGATACCTTGGGATGGGTGT 50.0 50.0 57.9 58.0 2321 bp 1.0 79.4
ndhD_p3 TAATGAGGCATGGGGATCCT TGATACCTTGGGATGGGTGT 50.0 50.0 57.9 58.0 2322 bp 1.0 79.4
ndhD_p4 TGAGGCATGGGGATCCTATT TGATACCTTGGGATGGGTGT 50.0 50.0 57.9 58.0 2319 bp 1.0 79.4
ndhD_p5 GCTAATGAGGCATGGGGATC TGATACCTTGGGATGGGTGT 55.0 50.0 58.2 58.0 2324 bp 1.0 79.1