Hotspot

ndhA

SSC · 125473-127742 · 2270 bp

Pi 0.00804

MarkerSeek score 63.10

Primer available yes

Label ndhA

Feature Metadata

label_name: ndhA
feature_type: gene
region: SSC
length_bp: 2270
variable_sites: 18
pi: 0.00804
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhA_p1	ACCATAGGAACCATAGCATGA	TGGGAGAAGTTGATCGGTGA	42.9	50.0	56.2	58.4	2359-2383 bp	1.0	56.3
ndhA_p2	ACCATAGGAACCATAGCATGAA	TGGGAGAAGTTGATCGGTGA	40.9	50.0	56.9	58.4	2359-2383 bp	1.0	54.2
ndhA_p3	ACCATAGGAACCATAGCATGA	ATGGGAGAAGTTGATCGGTG	42.9	50.0	56.2	57.3	2360-2384 bp	1.0	53.6
ndhA_p4	ACCATAGGAACCATAGCATGA	GGGAGAAGTTGATCGGTGAA	42.9	50.0	56.2	57.2	2358-2382 bp	1.0	53.1
ndhA_p5	TGTTTCTGTCTCTTGTTTGAACG	TGGGAGAAGTTGATCGGTGA	39.1	50.0	57.8	58.4	2319-2343 bp	1.0	53.1