Hotspot

trnN-GUU-ndhF

SSC · 114287-115153 · 867 bp

Back to Cyclea

Pi 0.0314
MarkerSeek score 67.50
Primer available yes
Label trnN-GUU-ndhF

Feature Metadata

label_name
trnN-GUU-ndhF
feature_type
igs
region
SSC
length_bp
867
variable_sites
26
pi
0.0314
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
67.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnN-GUU-ndhF_p1 AGGATTCGAACCTACGACCA GCGTTGGTGTTACGAGTTTC 50.0 50.0 58.2 57.7 1050-1066 bp 1.0 81.3
trnN-GUU-ndhF_p2 ATGGAACTTGGTTGTGGGTC GCGTTGGTGTTACGAGTTTC 50.0 50.0 58.0 57.7 1602-1618 bp 1.0 80.9
trnN-GUU-ndhF_p3 ATCCCGGATCTACATCGAGG GCGTTGGTGTTACGAGTTTC 55.0 50.0 58.2 57.7 1215-1231 bp 1.0 80.7
trnN-GUU-ndhF_p4 GGAACTTGGTTGTGGGTCTT GCGTTGGTGTTACGAGTTTC 50.0 50.0 57.9 57.7 1600-1616 bp 1.0 80.4
trnN-GUU-ndhF_p5 TGTTTTACTCCCCGCAACTC GCGTTGGTGTTACGAGTTTC 50.0 50.0 58.1 57.7 1470-1486 bp 1.0 80.3