Hotspot

rpl14-rpl16

LSC · 87085-87232 · 148 bp

Back to Cyclea

Pi 0.0203
MarkerSeek score 64.20
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
148
variable_sites
3
pi
0.0203
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 TAATTCTCTAGCCCCGCTGT TCAATTCGTTATTGCGGGGT 50.0 45.0 58.2 57.9 227-230 bp 1.0 78.4
rpl14-rpl16_p2 TAATTCTCTAGCCCCGCTGT AGCTGCGTCTAAAATGCCTA 50.0 45.0 58.2 57.3 254-257 bp 1.0 73.7
rpl14-rpl16_p3 TAATTCTCTAGCCCCGCTGT TAGCTGCGTCTAAAATGCCT 50.0 45.0 58.2 57.3 255-258 bp 1.0 73.7
rpl14-rpl16_p4 TAATTCTCTAGCCCCGCTGT CTCAATTCGTTATTGCGGGG 50.0 50.0 58.2 57.2 228-231 bp 1.0 73.3
rpl14-rpl16_p5 AACGTCACCGATATGAGCAT TCAATTCGTTATTGCGGGGT 45.0 45.0 57.1 57.9 284-287 bp 1.0 72.8