Hotspot

psbT-psbN

LSC · 80114-80172 · 59 bp

Back to Cyclea

Pi 0.0508
MarkerSeek score 77.20
Primer available yes
Label psbT-psbN

Feature Metadata

label_name
psbT-psbN
feature_type
igs
region
LSC
length_bp
59
variable_sites
3
pi
0.0508
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbT-psbN_p1 TTTTCGAGAACCGCCTAAGG GATCCATTCGAGGAACACGG 50.0 55.0 57.9 58.4 129 bp 1.0 77.7
psbT-psbN_p2 TTTTCGAGAACCGCCTAAGG GGCAACCCTCTCAACAACTA 50.0 50.0 57.9 57.4 152 bp 1.0 76.4
psbT-psbN_p3 TTTTCGAGAACCGCCTAAGG AGATCCATTCGAGGAACACG 50.0 50.0 57.9 57.4 130 bp 1.0 76.4
psbT-psbN_p4 CGCCTAAGGTTCCGACTAAA GATCCATTCGAGGAACACGG 50.0 55.0 57.3 58.4 118 bp 1.0 73.3
psbT-psbN_p5 CGCCTAAGGTTCCGACTAAA GGCAACCCTCTCAACAACTA 50.0 50.0 57.3 57.4 141 bp 1.0 71.9