Hotspot

trnE-UUC-trnT-GGU

LSC · 33741-34758 · 1018 bp

Back to Cyclea

Pi 0.00688
MarkerSeek score 62.70
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1018
variable_sites
7
pi
0.00688
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 CGTTGCCTCCTTGAAAGAGA GAACCGATGACTTACGCCTT 50.0 50.0 57.8 58.0 1122-1126 bp 1.0 79.1
trnE-UUC-trnT-GGU_p2 CGTTGCCTCCTTGAAAGAGA TACCGCTGAGTTAAAAGGGC 50.0 50.0 57.8 57.9 1087-1091 bp 1.0 78.3
trnE-UUC-trnT-GGU_p3 CGTTGCCTCCTTGAAAGAGA CCGATGACTTACGCCTTACC 50.0 55.0 57.8 58.2 1119-1123 bp 1.0 77.9
trnE-UUC-trnT-GGU_p4 CGTTGCCTCCTTGAAAGAGA TTGAACCGATGACTTACGCC 50.0 50.0 57.8 58.3 1124-1128 bp 1.0 76.9
trnE-UUC-trnT-GGU_p5 CGTTGCCTCCTTGAAAGAGA CGATGACTTACGCCTTACCA 50.0 50.0 57.8 57.4 1118-1122 bp 1.0 74.6