Hotspot

petN-psbM

LSC · 30456-31648 · 1193 bp

Back to Cyclea

Pi 0.00846
MarkerSeek score 54.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1193
variable_sites
10
pi
0.00846
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
54.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TTCACTCGTAGTATGGGGGC AGCTACTGCGCTGTTCATTC 55.0 50.0 58.9 58.6 1296-1306 bp 1.0 69.1
petN-psbM_p2 TTCACTCGTAGTATGGGGGC GCTACTGCGCTGTTCATTCT 55.0 50.0 58.9 58.6 1295-1305 bp 1.0 69.1
petN-psbM_p3 TTCACTCGTAGTATGGGGGC TAGCTACTGCGCTGTTCATT 55.0 45.0 58.9 57.3 1297-1307 bp 1.0 68.8
petN-psbM_p4 TTCACTCGTAGTATGGGGGC ACTGCGCTGTTCATTCTAGT 55.0 45.0 58.9 57.2 1292-1302 bp 1.0 68.2
petN-psbM_p5 TTCACTCGTAGTATGGGGGC ATAGCTACTGCGCTGTTCAT 55.0 45.0 58.9 57.1 1298-1308 bp 1.0 67.0