Hotspot

psbI-trnS-GCU

LSC · 9014-9169 · 156 bp

Back to Cyclea

Pi 0.0513
MarkerSeek score 77.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
156
variable_sites
8
pi
0.0513
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 AATCCTGGGCGTGAGGAATA TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.5 58.0 245 bp 1.0 78.4
psbI-trnS-GCU_p2 ATCCTGGGCGTGAGGAATAA TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.5 58.0 244 bp 1.0 78.4
psbI-trnS-GCU_p3 TAATCCTGGGCGTGAGGAAT TAAAGCGGCGGATTGCTAAT 50.0 45.0 58.5 58.0 246 bp 1.0 78.4
psbI-trnS-GCU_p4 AATCCTGGGCGTGAGGAATA AAACTGGGAGAGATGGCTGA 50.0 50.0 58.5 58.3 271 bp 1.0 75.7
psbI-trnS-GCU_p5 ATCCTGGGCGTGAGGAATAA AAACTGGGAGAGATGGCTGA 50.0 50.0 58.5 58.3 270 bp 1.0 75.7