Hotspot

trnH-GUG-psbA

LSC · 78-680 · 603 bp

Back to Cyclea

Pi 0.0138
MarkerSeek score 64.10
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
603
variable_sites
8
pi
0.0138
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.96
markerseek_score
64.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTGCCTTAATCCACTTGGCT CAACCGTGCTAACCTTGGTA 50.0 50.0 57.9 57.8 711-732 bp 1.0 81.0
trnH-GUG-psbA_p2 CTGCCTTAATCCACTTGGCT ATCAACCGTGCTAACCTTGG 50.0 50.0 57.9 58.2 713-734 bp 1.0 80.9
trnH-GUG-psbA_p3 ACTGCCTTAATCCACTTGGC CAACCGTGCTAACCTTGGTA 50.0 50.0 58.2 57.8 712-733 bp 1.0 80.8
trnH-GUG-psbA_p4 ACTGCCTTAATCCACTTGGC ATCAACCGTGCTAACCTTGG 50.0 50.0 58.2 58.2 714-735 bp 1.0 80.7
trnH-GUG-psbA_p5 CTGCCTTAATCCACTTGGCT CCGTGCTAACCTTGGTATGG 50.0 55.0 57.9 58.3 708-729 bp 1.0 79.7