Hotspot

rps8

LSC · 78539-78943 · 405 bp

Back to Cyclamen

Pi 0.0103

MarkerSeek score 47.30

Primer available yes

Label rps8

Feature Metadata

label_name: rps8
feature_type: gene
region: LSC
length_bp: 405
variable_sites: 8
pi: 0.0103
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps8_p1	AATGATACGCCCTCTGCTTG	GGGCTCGAGAATTGATGTGT	50.0	50.0	58.0	58.0	1249-1282 bp	1.0	84.9
rps8_p2	CCGGAACATAGCATTGGGAA	GGGCTCGAGAATTGATGTGT	50.0	50.0	57.9	58.0	1114-1147 bp	1.0	84.9
rps8_p3	CCGGAACATAGCATTGGGAA	GTAGCAGATAACAGTGGGGC	50.0	55.0	57.9	58.0	1130-1163 bp	1.0	84.8
rps8_p4	AATGATACGCCCTCTGCTTG	GTAGCAGATAACAGTGGGGC	50.0	55.0	58.0	58.0	1265-1298 bp	1.0	84.7
rps8_p5	AATGATACGCCCTCTGCTTG	GCCCGGGAATTGAGGTATTT	50.0	50.0	58.0	57.9	986-1019 bp	0.75	74.2