Hotspot

atpH

LSC · 13146-13391 · 246 bp

Back to Cyclamen

Pi 0.00203

MarkerSeek score 25.80

Primer available yes

Label atpH

Feature Metadata

label_name: atpH
feature_type: gene
region: LSC
length_bp: 246
variable_sites: 1
pi: 0.00203
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 25.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH_p1	CCTCATCCTCAAATCAGCCC	AGGCTGCTCGAAACAAAGAA	55.0	45.0	58.0	58.0	651-659 bp	1.0	83.4
atpH_p2	CCTCATCCTCAAATCAGCCC	TCCGTTTCGTTTGGGACTTT	55.0	45.0	58.0	58.0	714-722 bp	1.0	83.3
atpH_p3	CCTCATCCTCAAATCAGCCC	TTCCGTTTCGTTTGGGACTT	55.0	45.0	58.0	58.0	715-723 bp	1.0	83.3
atpH_p4	CGAATGGCCAGTATCCCAAA	AGGCTGCTCGAAACAAAGAA	50.0	45.0	57.9	58.0	932-945 bp	1.0	82.7
atpH_p5	CAGCCCTACATTCCTGGTTC	AGGCTGCTCGAAACAAAGAA	55.0	45.0	58.0	58.0	1217-1243 bp	1.0	82.6