Hotspot

psbL-psbF

LSC · 62790-62811 · 22 bp

Back to Cyclamen

Pi 0.0758

MarkerSeek score 74.90

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: LSC
length_bp: 22
variable_sites: 3
pi: 0.0758
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCAACATTTTGTTCGTTTGGGT	TCAGCAATGCAGTTCATCCA	36.4	45.0	57.5	57.8	84 bp	1.0	61.6
psbL-psbF_p2	TCAACATTTTGTTCGTTTGGGT	GCAATGCAGTTCATCCAACG	36.4	50.0	57.5	58.4	81 bp	1.0	60.3
psbL-psbF_p3	ACAGCAAGGACAAAAATGAGT	TCAGCAATGCAGTTCATCCA	38.1	45.0	56.3	57.8	138 bp	1.0	58.2
psbL-psbF_p4	CAACATTTTGTTCGTTTGGGT	TCAGCAATGCAGTTCATCCA	38.1	45.0	56.1	57.8	83 bp	1.0	57.9
psbL-psbF_p5	ACAGCAAGGACAAAAATGAGT	GCAATGCAGTTCATCCAACG	38.1	50.0	56.3	58.4	135 bp	1.0	56.9