Pi
0.0174
MarkerSeek score
65.40
Primer available
yes
Label
petA-psbJ
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| petA-psbJ_p1 | CGAAAAGGTTCAATTGTCCGA | TGGTTCATATTCTGGATTGGGT | 42.9 | 40.9 | 57.1 | 57.1 | 912-1144 bp | 1.0 | 47.0 |
| petA-psbJ_p2 | TCGAAAAGGTTCAATTGTCCG | TGGTTCATATTCTGGATTGGGT | 42.9 | 40.9 | 57.1 | 57.1 | 913-1145 bp | 1.0 | 47.0 |
| petA-psbJ_p3 | CGAAAAGGTTCAATTGTCCGA | ATTCTGGATTGGGTTCATCC | 42.9 | 45.0 | 57.1 | 55.1 | 904-1136 bp | 1.0 | 46.5 |
| petA-psbJ_p4 | TCGAAAAGGTTCAATTGTCCG | ATTCTGGATTGGGTTCATCC | 42.9 | 45.0 | 57.1 | 55.1 | 905-1137 bp | 1.0 | 46.5 |
| petA-psbJ_p5 | CGAAAAGGTTCAATTGTCCGA | TGGTTCATATTCTGGATTGGGTT | 42.9 | 39.1 | 57.1 | 57.8 | 912-1144 bp | 1.0 | 45.2 |