Pi
0.0174
MarkerSeek score
69.10
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | AATAAATCGCGCCAAAAGCC | ACCAATTTCGCCATATCCCC | 45.0 | 50.0 | 58.1 | 58.0 | 938-991 bp | 1.0 | 83.1 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 879-932 bp | 1.0 | 82.9 |
| trnT-UGU-trnL-UAA_p3 | AATAAATCGCGCCAAAAGCC | TAGCGTCTACCAATTTCGCC | 45.0 | 50.0 | 58.1 | 58.1 | 946-999 bp | 1.0 | 82.6 |
| trnT-UGU-trnL-UAA_p4 | GACCGTCGCATTACAAATGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 897-950 bp | 1.0 | 82.4 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 887-940 bp | 1.0 | 82.4 |