Hotspot

petN-psbM

LSC · 28079-29066 · 988 bp

Back to Cyclamen

Pi 0.0165

MarkerSeek score 65.60

Primer available yes

Label petN-psbM

Feature Metadata

label_name: petN-psbM
feature_type: igs
region: LSC
length_bp: 988
variable_sites: 32
pi: 0.0165
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-psbM_p1	ATGGGGAAGAAGTGGACTCT	ACTGCTTTTCTACTTATCATTTACGT	50.0	30.8	57.6	57.2	1034-1073 bp	1.0	46.4
petN-psbM_p2	ATGGGGAAGAAGTGGACTCT	CCTACTGCTTTTCTACTTATCA	50.0	36.4	57.6	53.0	1037-1076 bp	1.0	46.4
petN-psbM_p3	TAGTAAGTCTCGCTTGGGCT	ACTGCTTTTCTACTTATCATTTACGT	50.0	30.8	58.2	57.2	1095-1134 bp	1.0	46.3
petN-psbM_p4	TAGTAAGTCTCGCTTGGGCT	CCTACTGCTTTTCTACTTATCA	50.0	36.4	58.2	53.0	1098-1137 bp	1.0	46.3
petN-psbM_p5	TAGTAAGTCTCGCTTGGGCT	ACGTAAAAACAGTTAGTCAAAATCA	50.0	28.0	58.2	56.0	1073-1112 bp	1.0	46.3