Hotspot

atpF-atpH

LSC · 12747-13145 · 399 bp

Back to Cyclamen

Pi 0.0259

MarkerSeek score 68.90

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 399
variable_sites: 18
pi: 0.0259
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	CGAATGGCCAGTATCCCAAA	GGCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	491-500 bp	1.0	81.5
atpF-atpH_p2	ATTAAACCCGAAACTCCCGG	GGCTGGTTGTAGCATTAGCA	50.0	50.0	57.9	58.2	511-520 bp	1.0	80.8
atpF-atpH_p3	CGAATGGCCAGTATCCCAAA	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	493-502 bp	1.0	80.1
atpF-atpH_p4	ATTAAACCCGAAACTCCCGG	TGGGCTGGTTGTAGCATTAG	50.0	50.0	57.9	57.6	513-522 bp	1.0	79.4
atpF-atpH_p5	CGAATGGCCAGTATCCCAAA	CGGAGGGAAAAATACGAGGT	50.0	50.0	57.9	57.3	557-566 bp	1.0	77.5