Hotspot

trnH-GUG-psbA

LSC · 77-413 · 337 bp

Back to Cyclamen

Pi 0.0262

MarkerSeek score 63.60

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 337
variable_sites: 16
pi: 0.0262
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	CTGCCTTAATCCACTTGGCT	TGAAGCTCCATCTACAAATGG	50.0	42.9	57.9	55.7	390-480 bp	1.0	58.0
trnH-GUG-psbA_p2	ACTGCCTTAATCCACTTGGC	TGAAGCTCCATCTACAAATGG	50.0	42.9	58.2	55.7	391-481 bp	1.0	57.8
trnH-GUG-psbA_p3	AATCCACTTGGCTACATCCG	TGAAGCTCCATCTACAAATGG	50.0	42.9	57.7	55.7	383-473 bp	1.0	56.5
trnH-GUG-psbA_p4	CTGCCTTAATCCACTTGGCT	TTGAAGCTCCATCTACAAATGG	50.0	40.9	57.9	56.4	391-481 bp	1.0	56.0
trnH-GUG-psbA_p5	ACTGCCTTAATCCACTTGGC	TTGAAGCTCCATCTACAAATGG	50.0	40.9	58.2	56.4	392-482 bp	1.0	55.8