Hotspot

ycf1

SSC · 128473-133800 · 5328 bp

Pi 0.00333

MarkerSeek score 48.00

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5328
variable_sites: 157
pi: 0.00333
unique_haplotype_count: 48
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGCAGTCCGTTAAGATCGAA	GGTCTGGAATAGCACCACAG	50.0	55.0	57.7	58.0	247 bp	1.0	81.3
ycf1_p2	CGTACTGGCAGTCCGTTAAG	GGTCTGGAATAGCACCACAG	55.0	55.0	58.4	58.0	253 bp	1.0	80.9
ycf1_p3	TCTCTTCCCGTTCATTCTGC	GGTCTGGAATAGCACCACAG	50.0	55.0	57.6	58.0	273 bp	1.0	80.5
ycf1_p4	CTGGCAGTCCGTTAAGATCG	GGTCTGGAATAGCACCACAG	55.0	55.0	58.4	58.0	249 bp	1.0	80.1
ycf1_p5	GGCAGTCCGTTAAGATCGAA	AGATGAGTAAGCAACGGACG	50.0	50.0	57.7	57.7	214 bp	1.0	79.2