Hotspot

rps16

LSC · 4826-5951 · 1126 bp

Back to Cycas

Pi 0.00365
MarkerSeek score 60.80
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1126
variable_sites
33
pi
0.00365
unique_haplotype_count
51
primer_available
yes
alignment_reliability
1.00
markerseek_score
60.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 TGGGAACAGGAATGACTAAATGA TCGATCCCTGTTCCTATCGT 39.1 50.0 57.5 57.6 1292-1328 bp 1.0 54.5
rps16_p2 TGGGAACAGGAATGACTAAATGA TCGAATTCGATCCCTGTTCC 39.1 50.0 57.5 57.4 1298-1334 bp 1.0 52.6
rps16_p3 TGGGAACAGGAATGACTAAATGA CGAATTCGATCCCTGTTCCT 39.1 50.0 57.5 57.4 1297-1333 bp 1.0 52.6
rps16_p4 TGGGAACAGGAATGACTAAATG TCGATCCCTGTTCCTATCGT 40.9 50.0 56.1 57.6 1292-1328 bp 1.0 51.0
rps16_p5 TCTTCATATAAGATCGCATGGGA TCGATCCCTGTTCCTATCGT 39.1 50.0 57.0 57.6 1310-1346 bp 1.0 50.6