Hotspot

ndhF

SSC · 113657-115897 · 2241 bp

Pi 0.0031

MarkerSeek score 43.00

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2241
variable_sites: 63
pi: 0.0031
unique_haplotype_count: 38
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 43.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	AGGGGACGAAGAATTGCAAG	TCTAAAAATTACTCGATCTTCCT	50.0	30.4	58.2	53.0	2321-2337 bp	1.0	43.0
ndhF_p2	GAAGGGGACGAAGAATTGCA	TCTAAAAATTACTCGATCTTCCT	50.0	30.4	58.2	53.0	2323-2339 bp	1.0	43.0
ndhF_p3	GACGAAGAATTGCAAGGGGA	TCTAAAAATTACTCGATCTTCCT	50.0	30.4	58.2	53.0	2317-2333 bp	1.0	43.0
ndhF_p4	GGGAAGGGGACGAAGAATTG	TCTAAAAATTACTCGATCTTCCT	55.0	30.4	58.2	53.0	2325-2341 bp	1.0	43.0
ndhF_p5	ACAGAAAGAGAACAGGGGGA	TCTAAAAATTACTCGATCTTCCT	50.0	30.4	57.9	53.0	2363-2379 bp	1.0	42.9