Hotspot

chlL-trnN-GUU

IRa · 136398-137825 · 1428 bp

Back to Cycas

Pi 0.00256
MarkerSeek score 41.20
Primer available yes
Label chlL-trnN-GUU

Feature Metadata

label_name
chlL-trnN-GUU
feature_type
igs
region
IRa
length_bp
1428
variable_sites
29
pi
0.00256
unique_haplotype_count
27
primer_available
yes
alignment_reliability
1.00
markerseek_score
41.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
chlL-trnN-GUU_p1 TTCCGCCTTTTCCGTAAACT TGAACCTACGACCAGTCAGT 45.0 50.0 57.7 58.0 1515-1569 bp 1.0 79.8
chlL-trnN-GUU_p2 TTCCGCCTTTTCCGTAAACT GGAGCGAGCCCTTGAATAAA 45.0 50.0 57.7 58.0 1557-1611 bp 1.0 79.5
chlL-trnN-GUU_p3 TTCCGCCTTTTCCGTAAACT ACGACCAGTCAGTTAACAGC 45.0 50.0 57.7 57.8 1508-1562 bp 1.0 78.7
chlL-trnN-GUU_p4 TTCCGCCTTTTCCGTAAACT GTGGATCTCCCCAAGTAGGA 45.0 55.0 57.7 57.8 1537-1591 bp 1.0 78.4
chlL-trnN-GUU_p5 TTCCGCCTTTTCCGTAAACT AGTGGATCTCCCCAAGTAGG 45.0 55.0 57.7 57.8 1538-1592 bp 1.0 78.4