Hotspot

accD-psaI

LSC · 61028-61483 · 456 bp

Back to Cycas

Pi 0.00481
MarkerSeek score 34.90
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
456
variable_sites
16
pi
0.00481
unique_haplotype_count
15
primer_available
yes
alignment_reliability
1.00
markerseek_score
34.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 TCAACTTCAGAGAATTGTTCCTCA AAGGCACGAAAATCGAAGGT 37.5 45.0 58.1 58.1 533-553 bp 1.0 49.0
accD-psaI_p2 TCAACTTCAGAGAATTGTTCCTCA AGCCATTGTAATTGCCGGAA 37.5 45.0 58.1 58.2 568-588 bp 1.0 48.5
accD-psaI_p3 TCAACTTCAGAGAATTGTTCCTCA AAGCCATTGTAATTGCCGGA 37.5 45.0 58.1 58.2 569-589 bp 1.0 48.4
accD-psaI_p4 TCAACTTCAGAGAATTGTTCCTCA CCGCTAAAGGCACGAAAATC 37.5 50.0 58.1 57.8 539-559 bp 1.0 48.4
accD-psaI_p5 TCAACTTCAGAGAATTGTTCCTCA CGCTAAAGGCACGAAAATCG 37.5 50.0 58.1 58.2 538-558 bp 1.0 48.2