Hotspot

trnE-UUC-psbD

LSC · 31895-33339 · 1445 bp

Back to Cycas

Pi 0.00228
MarkerSeek score 55.90
Primer available yes
Label trnE-UUC-psbD

Feature Metadata

label_name
trnE-UUC-psbD
feature_type
igs
region
LSC
length_bp
1445
variable_sites
31
pi
0.00228
unique_haplotype_count
37
primer_available
yes
alignment_reliability
1.00
markerseek_score
55.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-psbD_p1 GCTGCCTCCTTGAAAGAGAG ATAGACCAGACCAACCCACA 55.0 50.0 58.3 58.0 1591-1620 bp 1.0 80.5
trnE-UUC-psbD_p2 GAAAGAGAGATGTCCTGGGC ATAGACCAGACCAACCCACA 55.0 50.0 57.7 58.0 1580-1609 bp 1.0 80.0
trnE-UUC-psbD_p3 GCTGCCTCCTTGAAAGAGAG GCCAGTCATCCGCAATATCA 55.0 50.0 58.3 58.1 1549-1578 bp 1.0 79.9
trnE-UUC-psbD_p4 GCTGCCTCCTTGAAAGAGAG AGACCAACCCACAAAAACGA 55.0 45.0 58.3 57.9 1584-1613 bp 1.0 79.7
trnE-UUC-psbD_p5 GCTGCCTCCTTGAAAGAGAG CACAAAAACGAAACGGTCCC 55.0 50.0 58.3 58.2 1575-1604 bp 1.0 79.4