Hotspot

trnS-GCU-ycf12

LSC · 9543-10231 · 689 bp

Back to Cycas

Pi 0.00418
MarkerSeek score 57.60
Primer available yes
Label trnS-GCU-ycf12

Feature Metadata

label_name
trnS-GCU-ycf12
feature_type
igs
region
LSC
length_bp
689
variable_sites
31
pi
0.00418
unique_haplotype_count
44
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-ycf12_p1 CGGAGAGAGAGGGATTCGAA AGTTACCTTTGCGAACTGCT 55.0 45.0 58.3 57.7 867-892 bp 1.0 79.8
trnS-GCU-ycf12_p2 ATTGAACGGAGAGAGAGGGA AGTTACCTTTGCGAACTGCT 50.0 45.0 57.5 57.7 873-898 bp 1.0 78.0
trnS-GCU-ycf12_p3 TGAACGGAGAGAGAGGGATT AGTTACCTTTGCGAACTGCT 50.0 45.0 57.5 57.7 871-896 bp 1.0 78.0
trnS-GCU-ycf12_p4 TTGAACGGAGAGAGAGGGAT AGTTACCTTTGCGAACTGCT 50.0 45.0 57.5 57.7 872-897 bp 1.0 78.0
trnS-GCU-ycf12_p5 AACGGAGAGAGAGGGATTCG AGTTACCTTTGCGAACTGCT 55.0 45.0 58.6 57.7 869-894 bp 1.0 77.5