Hotspot

psbA-trnK-UUU

LSC · 1063-1554 · 492 bp

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Pi 0.00738
MarkerSeek score 55.70
Primer available yes
Label psbA-trnK-UUU

Feature Metadata

label_name
psbA-trnK-UUU
feature_type
igs
region
LSC
length_bp
492
variable_sites
26
pi
0.00738
unique_haplotype_count
33
primer_available
yes
alignment_reliability
0.97
markerseek_score
55.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbA-trnK-UUU_p1 AAAACACCGAACCATCCGAT CACGGTTTGGGGAGAGATTT 45.0 50.0 57.8 57.8 664-705 bp 1.0 83.7
psbA-trnK-UUU_p2 AGTAATCCAGTCGCAGAAGC CACGGTTTGGGGAGAGATTT 50.0 50.0 57.7 57.8 623-664 bp 1.0 83.3
psbA-trnK-UUU_p3 AAAACACCGAACCATCCGAT GAAAGCCGTGTGCAATGAAA 45.0 45.0 57.8 57.9 692-733 bp 0.943396 81.7
psbA-trnK-UUU_p4 AGTAATCCAGTCGCAGAAGC GAAAGCCGTGTGCAATGAAA 50.0 45.0 57.7 57.9 651-692 bp 0.943396 81.2
psbA-trnK-UUU_p5 CACCGAACCATCCGATGTAA GAAAGCCGTGTGCAATGAAA 50.0 45.0 57.7 57.9 688-729 bp 0.943396 80.8