Hotspot

trnI-CAU

IRa · 149977-150050 · 74 bp

Back to Cyathula

Pi 0.0135
MarkerSeek score 60.30
Primer available yes
Label trnI-CAU

Feature Metadata

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnI-CAU_p1 AGACTTATTGGAGGGTCCCA TCACCATGTACGAGGATCCC 50.0 55.0 57.3 58.6 126 bp 1.0 70.6
trnI-CAU_p2 CTTATTGGAGGGTCCCATTGG TCACCATGTACGAGGATCCC 52.4 55.0 58.1 58.6 123 bp 1.0 67.5
trnI-CAU_p3 TTATTGGAGGGTCCCATTGG TCACCATGTACGAGGATCCC 50.0 55.0 56.9 58.6 122 bp 1.0 66.8
trnI-CAU_p4 AGACTTATTGGAGGGTCCCAT TCACCATGTACGAGGATCCC 47.6 55.0 57.8 58.6 126 bp 1.0 66.7
trnI-CAU_p5 AGACTTATTGGAGGGTCCCA ACCATGTACGAGGATCCCC 50.0 57.9 57.3 58.2 124 bp 1.0 66.0
trnI-CAU_p1 TCACCATGTACGAGGATCCC AGACTTATTGGAGGGTCCCA 55.0 50.0 58.6 57.3 126 bp 1.0 70.6
trnI-CAU_p2 TCACCATGTACGAGGATCCC CTTATTGGAGGGTCCCATTGG 55.0 52.4 58.6 58.1 123 bp 1.0 67.5
trnI-CAU_p3 TCACCATGTACGAGGATCCC TTATTGGAGGGTCCCATTGG 55.0 50.0 58.6 56.9 122 bp 1.0 66.8
trnI-CAU_p4 TCACCATGTACGAGGATCCC AGACTTATTGGAGGGTCCCAT 55.0 47.6 58.6 57.8 126 bp 1.0 66.7
trnI-CAU_p5 ACCATGTACGAGGATCCCC AGACTTATTGGAGGGTCCCA 57.9 50.0 58.2 57.3 124 bp 1.0 66.0