Hotspot

trnD-GUC

LSC · 30228-30301 · 74 bp

Back to Cyathula

Pi 0.0135

MarkerSeek score 60.30

Primer available yes

Label trnD-GUC

Feature Metadata

label_name: trnD-GUC
feature_type: tRNA
region: LSC
length_bp: 74
variable_sites: 1
pi: 0.0135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC_p1	CGTCCTTTTCCTAAAAAGCGG	TCACTCGTGTCTCTGTTACAAC	47.6	45.5	57.8	58.0	222 bp	1.0	54.5
trnD-GUC_p2	CGTCCTTTTCCTAAAAAGCGG	ACTCGTGTCTCTGTTACAACA	47.6	42.9	57.8	56.9	220 bp	1.0	54.2
trnD-GUC_p3	CGTCCTTTTCCTAAAAAGCGG	TCACTCGTGTCTCTGTTACA	47.6	45.0	57.8	55.9	222 bp	1.0	53.9
trnD-GUC_p4	CGTCCTTTTCCTAAAAAGCGG	ACTCGTGTCTCTGTTACAACAC	47.6	45.5	57.8	58.3	220 bp	1.0	52.4
trnD-GUC_p5	CGTCCTTTTCCTAAAAAGCGG	CACTCGTGTCTCTGTTACAACA	47.6	45.5	57.8	58.3	221 bp	1.0	52.4