Hotspot

rpoC2

LSC · 15867-19976 · 4110 bp

Back to Cyathula

Pi 0.000243

MarkerSeek score 54.00

Primer available yes

Label rpoC2

Feature Metadata

label_name: rpoC2
feature_type: gene
region: LSC
length_bp: 4110
variable_sites: 1
pi: 0.000243
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 54.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC2_p1	ACTACTTCGACAAGCCTTCG	TCGGGAGCTAGAGGAAATGT	50.0	50.0	57.7	57.8	230 bp	1.0	76.3
rpoC2_p2	ACTACTTCGACAAGCCTTCG	CGGGAGCTAGAGGAAATGTG	50.0	55.0	57.7	57.8	229 bp	1.0	75.7
rpoC2_p3	CTACTTCGACAAGCCTTCGC	TCGGGAGCTAGAGGAAATGT	55.0	50.0	59.0	57.8	229 bp	1.0	71.0
rpoC2_p4	CTACTTCGACAAGCCTTCGC	CGGGAGCTAGAGGAAATGTG	55.0	55.0	59.0	57.8	228 bp	1.0	70.4
rpoC2_p5	TTCTTTCCGGCATCATACCA	TCGGGAGCTAGAGGAAATGT	45.0	50.0	56.9	57.8	316 bp	1.0	70.2