Hotspot

psbA

LSC · 369-1430 · 1062 bp

Back to Cyathula

Pi 0

MarkerSeek score 40.00

Primer available yes

Label psbA

Feature Metadata

label_name: psbA
feature_type: gene
region: LSC
length_bp: 1062
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	ACCAACTTCTTGCCAGAACA	TGCTTGGGAGTCCCTGATAA	45.0	50.0	57.6	58.0	1190 bp	1.0	76.2
psbA_p2	ACCAACTTCTTGCCAGAACA	ATTCATGTGCTTGGGAGTCC	45.0	50.0	57.6	57.9	1197 bp	1.0	75.4
psbA_p3	ACCAACTTCTTGCCAGAACA	GGATTCATGTGCTTGGGAGT	45.0	50.0	57.6	57.9	1199 bp	1.0	75.4
psbA_p4	GAACAAGAAACCGGCATTGC	TGCTTGGGAGTCCCTGATAA	50.0	50.0	58.6	58.0	1175 bp	1.0	75.1
psbA_p5	GGCATTGCCCCTTTACTTTC	TGCTTGGGAGTCCCTGATAA	50.0	50.0	57.3	58.0	1163 bp	1.0	74.3