Hotspot

petD

LSC · 75619-76865 · 1247 bp

Back to Cyathula

Pi 0.000802

MarkerSeek score 59.20

Primer available yes

Label petD

Feature Metadata

label_name: petD
feature_type: gene
region: LSC
length_bp: 1247
variable_sites: 1
pi: 0.000802
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD_p1	ACTGGTTGAAGGTAATTCTCTGA	ACTTTGAAGCGACTATTCCCT	39.1	42.9	57.2	57.0	1350 bp	1.0	40.1
petD_p2	TCACTGGTTGAAGGTAATTCTCT	ACTTTGAAGCGACTATTCCCT	39.1	42.9	57.2	57.0	1352 bp	1.0	40.1
petD_p3	ACTGGTTGAAGGTAATTCTCTGA	CACTTTGAAGCGACTATTCCC	39.1	47.6	57.2	57.0	1351 bp	1.0	40.1
petD_p4	TCACTGGTTGAAGGTAATTCTCT	CACTTTGAAGCGACTATTCCC	39.1	47.6	57.2	57.0	1353 bp	1.0	40.1
petD_p5	CACTGGTTGAAGGTAATTCTCTG	ACTTTGAAGCGACTATTCCCT	43.5	42.9	57.1	57.0	1351 bp	1.0	40.1