Hotspot

trnN-GUU-ndhF

IRb · 106792-108884 · 2093 bp

Back to Cyathula

Pi 0.00222

MarkerSeek score 47.20

Primer available yes

Label trnN-GUU-ndhF

Feature Metadata

label_name: trnN-GUU-ndhF
feature_type: igs
region: IRb
length_bp: 2093
variable_sites: 4
pi: 0.00222
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.86
markerseek_score: 47.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnN-GUU-ndhF_p1	GCAACGCAACCAGCATATTT	GTATGTAGGAGGGGGTCGAA	45.0	55.0	58.0	57.9	2382-2684 bp	1.0	83.5
trnN-GUU-ndhF_p2	GCAACGCAACCAGCATATTT	AAGTATGTAGGAGGGGGTCG	45.0	55.0	58.0	57.9	2384-2686 bp	1.0	83.4
trnN-GUU-ndhF_p3	GGCAGAATAGCAGAGCAAGT	GTATGTAGGAGGGGGTCGAA	50.0	55.0	58.0	57.9	2321-2623 bp	1.0	83.3
trnN-GUU-ndhF_p4	GCGAGCCCCTTCATTCTTTA	GTATGTAGGAGGGGGTCGAA	50.0	55.0	58.0	57.9	1998-2300 bp	1.0	83.3
trnN-GUU-ndhF_p5	GGCAGAATAGCAGAGCAAGT	AAGTATGTAGGAGGGGGTCG	50.0	55.0	58.0	57.9	2323-2625 bp	1.0	83.2