Hotspot

matK-trnK-UUU

LSC · 3435-4159 · 725 bp

Back to Cyathula

Pi 0

MarkerSeek score 40.00

Primer available yes

Label matK-trnK-UUU

Feature Metadata

label_name: matK-trnK-UUU
feature_type: igs
region: LSC
length_bp: 725
variable_sites: 0
pi: 0
unique_haplotype_count: 1
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 40.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
matK-trnK-UUU_p1	TGTTGCCAAGACCTATCTAGT	AACGGTAGAGTACTCGGCTT	42.9	50.0	56.1	58.2	796 bp	1.0	55.3
matK-trnK-UUU_p2	TGTTGCCAAGACCTATCTAGT	ACGGTAGAGTACTCGGCTTT	42.9	50.0	56.1	58.2	795 bp	1.0	55.3
matK-trnK-UUU_p3	TGTTGCCAAGACCTATCTAGTTC	AACGGTAGAGTACTCGGCTT	43.5	50.0	57.9	58.2	796 bp	1.0	54.0
matK-trnK-UUU_p4	TGTTGCCAAGACCTATCTAGTTC	ACGGTAGAGTACTCGGCTTT	43.5	50.0	57.9	58.2	795 bp	1.0	54.0
matK-trnK-UUU_p5	TGCCAAGACCTATCTAGTTCTCT	ACGGTAGAGTACTCGGCTTT	43.5	50.0	58.1	58.2	792 bp	1.0	53.6