primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA_p1	psbA	1	ACCAACTTCTTGCCAGAACA	TGCTTGGGAGTCCCTGATAA	20	20	45.0	50.0	57.6	58.0	0.0	28.7	0.469958	369	1430	1190-1190	1.000000	76.2
psbA_p2	psbA	2	ACCAACTTCTTGCCAGAACA	ATTCATGTGCTTGGGAGTCC	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.570161	369	1430	1197-1197	1.000000	75.4
psbA_p3	psbA	3	ACCAACTTCTTGCCAGAACA	GGATTCATGTGCTTGGGAGT	20	20	45.0	50.0	57.6	57.9	0.0	0.0	0.570161	369	1430	1199-1199	1.000000	75.4
psbA_p4	psbA	4	GAACAAGAAACCGGCATTGC	TGCTTGGGAGTCCCTGATAA	20	20	50.0	50.0	58.6	58.0	0.0	28.7	0.616213	369	1430	1175-1175	1.000000	75.1
psbA_p5	psbA	5	GGCATTGCCCCTTTACTTTC	TGCTTGGGAGTCCCTGATAA	20	20	50.0	50.0	57.3	58.0	17.3	28.7	0.707547	369	1430	1163-1163	1.000000	74.3
matK_p1	matK	1	TACTCAATGGCTTCAACCCC	CCTTGTTTTGACTGTATCGCA	20	21	50.0	42.9	57.5	56.8	0.0	0.0	2.674894	1923	3434	1710-1710	1.000000	58.6
matK_p2	matK	2	TACTCAATGGCTTCAACCCC	ACCTTGTTTTGACTGTATCGC	20	21	50.0	42.9	57.5	56.8	0.0	0.0	2.676928	1923	3434	1711-1711	1.000000	58.6
matK_p3	matK	3	TACTCAATGGCTTCAACCCC	CCTTGTTTTGACTGTATCGCAC	20	22	50.0	45.5	57.5	58.2	0.0	0.0	2.697034	1923	3434	1710-1710	1.000000	58.4
matK_p4	matK	4	CTCAATGGCTTCAACCCCTA	CCTTGTTTTGACTGTATCGCA	20	21	50.0	42.9	57.2	56.8	0.0	0.0	2.974432	1923	3434	1708-1708	1.000000	56.2
matK_p5	matK	5	CTCAATGGCTTCAACCCCTA	ACCTTGTTTTGACTGTATCGC	20	21	50.0	42.9	57.2	56.8	0.0	0.0	2.976466	1923	3434	1709-1709	1.000000	56.2
matK-trnK-UUU_p1	matK-trnK-UUU	1	TGTTGCCAAGACCTATCTAGT	AACGGTAGAGTACTCGGCTT	21	20	42.9	50.0	56.1	58.2	0.0	17.1	3.091963	3435	4159	796-796	1.000000	55.3
matK-trnK-UUU_p2	matK-trnK-UUU	2	TGTTGCCAAGACCTATCTAGT	ACGGTAGAGTACTCGGCTTT	21	20	42.9	50.0	56.1	58.2	0.0	17.1	3.091963	3435	4159	795-795	1.000000	55.3
matK-trnK-UUU_p3	matK-trnK-UUU	3	TGTTGCCAAGACCTATCTAGTTC	AACGGTAGAGTACTCGGCTT	23	20	43.5	50.0	57.9	58.2	0.0	17.1	3.254523	3435	4159	796-796	1.000000	54.0
matK-trnK-UUU_p4	matK-trnK-UUU	4	TGTTGCCAAGACCTATCTAGTTC	ACGGTAGAGTACTCGGCTTT	23	20	43.5	50.0	57.9	58.2	0.0	17.1	3.254523	3435	4159	795-795	1.000000	54.0
matK-trnK-UUU_p5	matK-trnK-UUU	5	TGCCAAGACCTATCTAGTTCTCT	ACGGTAGAGTACTCGGCTTT	23	20	43.5	50.0	58.1	58.2	0.0	17.1	3.295410	3435	4159	792-792	1.000000	53.6
trnG-UCC_p1	trnG-UCC	1	TCAGTGTTCGACAAAAGTTCT	TCGGATTGTATGTGATGTGAGA	21	22	38.1	40.9	55.7	57.0	0.0	0.0	6.297143	8626	9406	917-917	1.000000	40.0
trnG-UCC_p2	trnG-UCC	2	TTCAGTGTTCGACAAAAGTTCT	TCGGATTGTATGTGATGTGAGA	22	22	36.4	40.9	56.4	57.0	0.0	0.0	6.553644	8626	9406	918-918	1.000000	40.0
trnG-UCC_p3	trnG-UCC	3	ACATCAAATTTCAGTGTTCGACA	TCGGATTGTATGTGATGTGAGA	23	22	34.8	40.9	57.3	57.0	0.0	0.0	6.750152	8626	9406	927-927	1.000000	40.0
trnG-UCC_p4	trnG-UCC	4	TCAGTGTTCGACAAAAGTTCT	CGGATTGTATGTGATGTGAGA	21	21	38.1	42.9	55.7	55.5	0.0	0.0	6.783359	8626	9406	916-916	1.000000	40.0
trnG-UCC_p5	trnG-UCC	5	TCAGTGTTCGACAAAAGTTCT	TCGGATTGTATGTGATGTGAG	21	21	38.1	42.9	55.7	55.5	0.0	0.0	6.783359	8626	9406	917-917	1.000000	40.0
trnR-UCU_p1	trnR-UCU	1	TCTCACATCACATACAATCCGA	TCAAGAACAGAAGGAACGCT	22	20	40.9	45.0	57.0	57.1	0.0	0.0	3.911243	9545	9616	247-247	1.000000	49.0
trnR-UCU_p2	trnR-UCU	2	TCTCACATCACATACAATCCGA	TCAAGAACAGAAGGAACGCTT	22	21	40.9	42.9	57.0	57.8	0.0	0.0	4.194560	9545	9616	247-247	1.000000	46.8
trnR-UCU_p3	trnR-UCU	3	TCTCACATCACATACAATCCGA	TTCAAGAACAGAAGGAACGCT	22	21	40.9	42.9	57.0	57.8	0.0	0.0	4.194560	9545	9616	248-248	1.000000	46.8
trnR-UCU_p4	trnR-UCU	4	TCTCACATCACATACAATCCGA	GGAACGCTTTCTACTTCAGGA	22	21	40.9	47.6	57.0	57.7	0.0	2.7	4.315096	9545	9616	235-235	1.000000	45.8
trnR-UCU_p5	trnR-UCU	5	TCTCACATCACATACAATCCGA	AGGAACGCTTTCTACTTCAGG	22	21	40.9	47.6	57.0	57.7	0.0	2.7	4.321229	9545	9616	236-236	1.000000	45.8
rpoC2_p1	rpoC2	1	ACTACTTCGACAAGCCTTCG	TCGGGAGCTAGAGGAAATGT	20	20	50.0	50.0	57.7	57.8	0.0	0.0	0.510018	19369	19488	230-230	1.000000	76.3
rpoC2_p2	rpoC2	2	ACTACTTCGACAAGCCTTCG	CGGGAGCTAGAGGAAATGTG	20	20	50.0	55.0	57.7	57.8	0.0	0.0	0.580512	19369	19488	229-229	1.000000	75.7
rpoC2_p3	rpoC2	3	CTACTTCGACAAGCCTTCGC	TCGGGAGCTAGAGGAAATGT	20	20	55.0	50.0	59.0	57.8	0.0	0.0	1.167919	19369	19488	229-229	1.000000	71.0
rpoC2_p4	rpoC2	4	CTACTTCGACAAGCCTTCGC	CGGGAGCTAGAGGAAATGTG	20	20	55.0	55.0	59.0	57.8	0.0	0.0	1.238413	19369	19488	228-228	1.000000	70.4
rpoC2_p5	rpoC2	5	TTCTTTCCGGCATCATACCA	TCGGGAGCTAGAGGAAATGT	20	20	45.0	50.0	56.9	57.8	0.0	0.0	1.251819	19369	19488	316-316	1.000000	70.2
rpoC1_p1	rpoC1	1	AAAGACTCGAATTCTGCCGA	ACGATCTTTGGCTCTGGAAC	20	20	45.0	50.0	57.3	57.9	3.5	0.0	0.844240	20167	22967	2965-2966	1.000000	73.3
rpoC1_p2	rpoC1	2	AAGACTCGAATTCTGCCGAT	ACGATCTTTGGCTCTGGAAC	20	20	45.0	50.0	57.0	57.9	3.5	0.0	1.070233	20167	22967	2964-2965	1.000000	71.5
rpoC1_p3	rpoC1	3	AAAGACTCGAATTCTGCCGA	CGATCTTTGGCTCTGGAACT	20	20	45.0	50.0	57.3	57.6	3.5	0.0	1.136907	20167	22967	2964-2965	1.000000	70.9
rpoC1_p4	rpoC1	4	GAAAGACTCGAATTCTGCCG	ACGATCTTTGGCTCTGGAAC	20	20	50.0	50.0	56.9	57.9	3.5	0.0	1.175785	20167	22967	2966-2967	1.000000	70.6
rpoC1_p5	rpoC1	5	AAAGACTCGAATTCTGCCGA	GCTCGTTCGAGAACTACGAT	20	20	45.0	50.0	57.3	57.5	3.5	23.5	1.198931	20167	22967	2980-2981	1.000000	70.4
psbM-trnD-GUC_p1	psbM-trnD-GUC	1	TCATTTTGGCTGACCGTTTT	GTTCGAGCCCCGTCAGTC	20	18	40.0	66.7	56.4	60.1	0.0	0.0	5.683401	29232	30227	1118-1118	1.000000	40.0
psbM-trnD-GUC_p2	psbM-trnD-GUC	2	AATCATTTTGGCTGACCGTT	GTTCGAGCCCCGTCAGTC	20	18	40.0	66.7	56.2	60.1	0.0	0.0	5.914373	29232	30227	1120-1120	1.000000	40.0
psbM-trnD-GUC_p3	psbM-trnD-GUC	3	ATCATTTTGGCTGACCGTTT	GTTCGAGCCCCGTCAGTC	20	18	40.0	66.7	56.2	60.1	0.0	0.0	5.914373	29232	30227	1119-1119	1.000000	40.0
psbM-trnD-GUC_p4	psbM-trnD-GUC	4	TCATTTTGGCTGACCGTTTTT	GTTCGAGCCCCGTCAGTC	21	18	38.1	66.7	57.2	60.1	0.0	0.0	5.936341	29232	30227	1118-1118	1.000000	40.0
psbM-trnD-GUC_p5	psbM-trnD-GUC	5	TTTGGCTGACCGTTTTTACA	GTTCGAGCCCCGTCAGTC	20	18	40.0	66.7	56.1	60.1	0.0	0.0	6.032971	29232	30227	1114-1114	1.000000	40.0
trnD-GUC_p1	trnD-GUC	1	CGTCCTTTTCCTAAAAAGCGG	TCACTCGTGTCTCTGTTACAAC	21	22	47.6	45.5	57.8	58.0	5.3	0.0	3.236252	30228	30301	222-222	1.000000	54.5
trnD-GUC_p2	trnD-GUC	2	CGTCCTTTTCCTAAAAAGCGG	ACTCGTGTCTCTGTTACAACA	21	21	47.6	42.9	57.8	56.9	5.3	0.0	3.266956	30228	30301	220-220	1.000000	54.2
trnD-GUC_p3	trnD-GUC	3	CGTCCTTTTCCTAAAAAGCGG	TCACTCGTGTCTCTGTTACA	21	20	47.6	45.0	57.8	55.9	5.3	0.0	3.311108	30228	30301	222-222	1.000000	53.9
trnD-GUC_p4	trnD-GUC	4	CGTCCTTTTCCTAAAAAGCGG	ACTCGTGTCTCTGTTACAACAC	21	22	47.6	45.5	57.8	58.3	5.3	8.3	3.494014	30228	30301	220-220	1.000000	52.4
trnD-GUC_p5	trnD-GUC	5	CGTCCTTTTCCTAAAAAGCGG	CACTCGTGTCTCTGTTACAACA	21	22	47.6	45.5	57.8	58.3	5.3	0.0	3.495104	30228	30301	221-221	1.000000	52.4
psbZ-trnG-GCC_p1	psbZ-trnG-GCC	1	TGTATTTGCTTCTCCCGACG	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.0	57.8	0.0	1.1	0.174415	35775	36082	470-470	1.000000	78.8
psbZ-trnG-GCC_p2	psbZ-trnG-GCC	2	CGACGGTTGGTCAAGTAACA	CGTCTTCTCCTTGGCAAAGA	20	20	50.0	50.0	58.1	57.8	0.0	1.1	0.314319	35775	36082	455-455	1.000000	77.7
psbZ-trnG-GCC_p3	psbZ-trnG-GCC	3	GTATTTGCTTCTCCCGACGG	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.7	57.8	7.0	1.1	0.882408	35775	36082	469-469	1.000000	73.1
psbZ-trnG-GCC_p4	psbZ-trnG-GCC	4	CCGACGGTTGGTCAAGTAAC	CGTCTTCTCCTTGGCAAAGA	20	20	55.0	50.0	58.9	57.8	5.5	1.1	1.027358	35775	36082	456-456	1.000000	72.0
psbZ-trnG-GCC_p5	psbZ-trnG-GCC	5	TGTATTTGCTTCTCCCGACG	CGGATAGCGGGAATCGAAC	20	19	50.0	57.9	58.0	58.2	0.0	0.0	1.194097	35775	36082	492-492	1.000000	70.6
petB_p1	petB	1	CCCTTTTGGTAGTTCGATCGT	ACCGTTGTTCTTCTCCCAAG	21	20	47.6	50.0	58.0	57.7	15.9	0.0	1.287442	74026	75414	1547-1547	1.000000	69.7
petB_p2	petB	2	CCCTTTTGGTAGTTCGATCGT	CCGTTGTTCTTCTCCCAAGT	21	20	47.6	50.0	58.0	57.7	15.9	0.0	1.287442	74026	75414	1546-1546	1.000000	69.7
petB_p3	petB	3	TTGGTAGTTCGATCGTGGAA	ACCGTTGTTCTTCTCCCAAG	20	20	45.0	50.0	56.6	57.7	15.9	0.0	1.657417	74026	75414	1542-1542	1.000000	66.7
petB_p4	petB	4	TTGGTAGTTCGATCGTGGAA	CCGTTGTTCTTCTCCCAAGT	20	20	45.0	50.0	56.6	57.7	15.9	0.0	1.657417	74026	75414	1541-1541	1.000000	66.7
petB_p5	petB	5	TTTGGTAGTTCGATCGTGGA	CCGTTGTTCTTCTCCCAAGT	20	20	45.0	50.0	56.6	57.7	15.9	0.0	1.657417	74026	75414	1542-1542	1.000000	66.7
petD_p1	petD	1	ACTGGTTGAAGGTAATTCTCTGA	ACTTTGAAGCGACTATTCCCT	23	21	39.1	42.9	57.2	57.0	0.0	0.0	5.808636	75619	76865	1350-1350	1.000000	40.1
petD_p2	petD	2	TCACTGGTTGAAGGTAATTCTCT	ACTTTGAAGCGACTATTCCCT	23	21	39.1	42.9	57.2	57.0	0.0	0.0	5.808636	75619	76865	1352-1352	1.000000	40.1
petD_p3	petD	3	ACTGGTTGAAGGTAATTCTCTGA	CACTTTGAAGCGACTATTCCC	23	21	39.1	47.6	57.2	57.0	0.0	0.0	5.844041	75619	76865	1351-1351	1.000000	40.1
petD_p4	petD	4	TCACTGGTTGAAGGTAATTCTCT	CACTTTGAAGCGACTATTCCC	23	21	39.1	47.6	57.2	57.0	0.0	0.0	5.844041	75619	76865	1353-1353	1.000000	40.1
petD_p5	petD	5	CACTGGTTGAAGGTAATTCTCTG	ACTTTGAAGCGACTATTCCCT	23	21	43.5	42.9	57.1	57.0	0.0	0.0	5.852797	75619	76865	1351-1351	1.000000	40.1
rpl16_p1	rpl16	1	ACTGTTTGGCCAATTGTCCA	AGAATGCCAACTTTGTTCATTCT	20	23	45.0	34.8	58.2	57.2	21.3	20.0	4.009487	80320	81723	1581-1581	1.000000	48.0
rpl16_p2	rpl16	2	ACTGTTTGGCCAATTGTCCA	AAGAATGCCAACTTTGTTCA	20	20	45.0	35.0	58.2	53.9	21.3	0.0	4.331497	80320	81723	1582-1582	1.000000	45.4
rpl16_p3	rpl16	3	ACTGTTTGGCCAATTGTCCA	TGCCAACTTTGTTCATTCTT	20	20	45.0	35.0	58.2	53.9	21.3	0.0	4.331497	80320	81723	1577-1577	1.000000	45.4
rpl16_p4	rpl16	4	ACTGTTTGGCCAATTGTCCA	AAGAATGCCAACTTTGTTCATTCT	20	24	45.0	33.3	58.2	57.8	21.3	20.0	4.378762	80320	81723	1582-1582	1.000000	45.0
rpl16_p5	rpl16	5	ACTGTTTGGCCAATTGTCCA	AGAATGCCAACTTTGTTCATTCTT	20	24	45.0	33.3	58.2	57.8	21.3	20.0	4.378762	80320	81723	1581-1581	1.000000	45.0
trnI-CAU_p1	trnI-CAU	1	AGACTTATTGGAGGGTCCCA	TCACCATGTACGAGGATCCC	20	20	50.0	55.0	57.3	58.6	11.6	8.6	1.251172	84860	84933	126-126	1.000000	70.6
trnI-CAU_p2	trnI-CAU	2	CTTATTGGAGGGTCCCATTGG	TCACCATGTACGAGGATCCC	21	20	52.4	55.0	58.1	58.6	11.6	8.6	1.640548	84860	84933	123-123	1.000000	67.5
trnI-CAU_p3	trnI-CAU	3	TTATTGGAGGGTCCCATTGG	TCACCATGTACGAGGATCCC	20	20	50.0	55.0	56.9	58.6	11.6	8.6	1.733310	84860	84933	122-122	1.000000	66.8
trnI-CAU_p4	trnI-CAU	4	AGACTTATTGGAGGGTCCCAT	TCACCATGTACGAGGATCCC	21	20	47.6	55.0	57.8	58.6	11.6	8.6	1.741538	84860	84933	126-126	1.000000	66.7
trnI-CAU_p5	trnI-CAU	5	AGACTTATTGGAGGGTCCCA	ACCATGTACGAGGATCCCC	20	19	50.0	57.9	57.3	58.2	11.6	8.6	1.825156	84860	84933	124-124	1.000000	66.0
trnN-GUU-ndhF_p1	trnN-GUU-ndhF	1	GCAACGCAACCAGCATATTT	GTATGTAGGAGGGGGTCGAA	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.082771	106792	108884	2382-2684	1.000000	83.5
trnN-GUU-ndhF_p2	trnN-GUU-ndhF	2	GCAACGCAACCAGCATATTT	AAGTATGTAGGAGGGGGTCG	20	20	45.0	55.0	58.0	57.9	0.0	0.0	0.088712	106792	108884	2384-2686	1.000000	83.4
trnN-GUU-ndhF_p3	trnN-GUU-ndhF	3	GGCAGAATAGCAGAGCAAGT	GTATGTAGGAGGGGGTCGAA	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.113217	106792	108884	2321-2623	1.000000	83.3
trnN-GUU-ndhF_p4	trnN-GUU-ndhF	4	GCGAGCCCCTTCATTCTTTA	GTATGTAGGAGGGGGTCGAA	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.116074	106792	108884	1998-2300	1.000000	83.3
trnN-GUU-ndhF_p5	trnN-GUU-ndhF	5	GGCAGAATAGCAGAGCAAGT	AAGTATGTAGGAGGGGGTCG	20	20	50.0	55.0	58.0	57.9	0.0	0.0	0.119158	106792	108884	2323-2625	1.000000	83.2
ndhF_p1	ndhF	1	AATTTGCTGGGGAATCGACA	TCTTTCAAAAGAGGCAAGAGC	20	21	45.0	42.9	57.8	56.7	0.0	1.1	2.486289	108885	111122	2430-2430	0.500000	40.1
ndhF_p2	ndhF	2	TTTGCTGGGGAATCGACATT	TCTTTCAAAAGAGGCAAGAGC	20	21	45.0	42.9	57.8	56.7	0.0	1.1	2.486289	108885	111122	2428-2428	0.500000	40.1
ndhF_p3	ndhF	3	GCTGGGGAATCGACATTGAA	TCTTTCAAAAGAGGCAAGAGC	20	21	50.0	42.9	58.2	56.7	0.0	1.1	2.521720	108885	111122	2425-2425	0.500000	39.8
ndhF_p4	ndhF	4	ATTTGCTGGGGAATCGACAT	TCTTTCAAAAGAGGCAAGAGC	20	21	45.0	42.9	57.6	56.7	0.0	1.1	2.714150	108885	111122	2429-2429	0.500000	38.3
ndhF_p5	ndhF	5	TCAATTTGCTGGGGAATCGA	TCTTTCAAAAGAGGCAAGAGC	20	21	45.0	42.9	57.5	56.7	0.0	1.1	2.777563	108885	111122	2432-2432	0.500000	37.8
rps15_p1	rps15	1	ACAGTCATAGGTTTTTCCCCG	ACCAAATCAAAATAGCTGGCA	21	21	47.6	38.1	57.9	56.4	0.0	0.0	3.681177	121793	122065	483-483	1.000000	50.7
rps15_p2	rps15	2	CAGTCATAGGTTTTTCCCCGA	ACCAAATCAAAATAGCTGGCA	21	21	47.6	38.1	57.7	56.4	0.0	0.0	3.957434	121793	122065	482-482	1.000000	48.5
rps15_p3	rps15	3	ACAGTCATAGGTTTTTCCCCG	ACCAAATCAAAATAGCTGGCAT	21	22	47.6	36.4	57.9	56.9	0.0	0.0	4.166463	121793	122065	483-483	1.000000	46.8
rps15_p4	rps15	4	CAGTCATAGGTTTTTCCCCGA	ACCAAATCAAAATAGCTGGCAT	21	22	47.6	36.4	57.7	56.9	0.0	0.0	4.442720	121793	122065	482-482	1.000000	44.6
rps15_p5	rps15	5	AGTCATAGGTTTTTCCCCGA	ACCAAATCAAAATAGCTGGCA	20	21	45.0	38.1	56.1	56.4	0.0	0.0	4.472694	121793	122065	481-481	1.000000	44.4
trnI-CAU_p1	trnI-CAU	1	TCACCATGTACGAGGATCCC	AGACTTATTGGAGGGTCCCA	20	20	55.0	50.0	58.6	57.3	8.6	11.6	1.251172	149977	150050	126-126	1.000000	70.6
trnI-CAU_p2	trnI-CAU	2	TCACCATGTACGAGGATCCC	CTTATTGGAGGGTCCCATTGG	20	21	55.0	52.4	58.6	58.1	8.6	11.6	1.640548	149977	150050	123-123	1.000000	67.5
trnI-CAU_p3	trnI-CAU	3	TCACCATGTACGAGGATCCC	TTATTGGAGGGTCCCATTGG	20	20	55.0	50.0	58.6	56.9	8.6	11.6	1.733310	149977	150050	122-122	1.000000	66.8
trnI-CAU_p4	trnI-CAU	4	TCACCATGTACGAGGATCCC	AGACTTATTGGAGGGTCCCAT	20	21	55.0	47.6	58.6	57.8	8.6	11.6	1.741538	149977	150050	126-126	1.000000	66.7
trnI-CAU_p5	trnI-CAU	5	ACCATGTACGAGGATCCCC	AGACTTATTGGAGGGTCCCA	19	20	57.9	50.0	58.2	57.3	8.6	11.6	1.825156	149977	150050	124-124	1.000000	66.0