Hotspot

psbZ

Genome · 41301-41489 · 189 bp

Back to Cyathodium

Pi 0.00529

MarkerSeek score 55.50

Primer available yes

Label psbZ

Feature Metadata

label_name: psbZ
feature_type: gene
region: Genome
length_bp: 189
variable_sites: 1
pi: 0.00529
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 55.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ_p1	ACAGGATTTGATGGTTCAACA	ACTTGTTCAAAATGTTGTTAAACT	38.1	25.0	55.7	54.3	322 bp	1.0	40.2
psbZ_p2	AACAGGATTTGATGGTTCAACA	ACTTGTTCAAAATGTTGTTAAACT	36.4	25.0	56.4	54.3	323 bp	1.0	40.2
psbZ_p3	ACAGGATTTGATGGTTCAACA	ACTTGTTCAAAATGTTGTTAAAC	38.1	26.1	55.7	52.9	322 bp	1.0	40.2
psbZ_p4	ACAGGATTTGATGGTTCAACAT	ACTTGTTCAAAATGTTGTTAAACT	36.4	25.0	56.2	54.3	322 bp	1.0	40.2
psbZ_p5	AAACAGGATTTGATGGTTCAACA	ACTTGTTCAAAATGTTGTTAAACT	34.8	25.0	57.1	54.3	324 bp	1.0	40.2