Hotspot

psbB

Genome · 68188-69714 · 1527 bp

Back to Cyathodium

Pi 0.000655

MarkerSeek score 59.10

Primer available yes

Label psbB

Feature Metadata

label_name: psbB
feature_type: gene
region: Genome
length_bp: 1527
variable_sites: 1
pi: 0.000655
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 59.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbB_p1	ACTCCGCATCTAGGATGGTT	TCGATCATTCTGAAACATCAAAGT	50.0	33.3	58.2	57.0	1706 bp	1.0	40.0
psbB_p2	ACTCCGCATCTAGGATGGTT	ACAAATCGATCATTCTGAAACATCA	50.0	32.0	58.2	57.6	1711 bp	1.0	40.0
psbB_p3	ACTCCGCATCTAGGATGGTT	CGATCATTCTGAAACATCAAAGT	50.0	34.8	58.2	55.6	1705 bp	1.0	40.0
psbB_p4	ACTCCGCATCTAGGATGGTT	TCGATCATTCTGAAACATCAAAGTT	50.0	32.0	58.2	57.6	1706 bp	1.0	40.0
psbB_p5	TACTCCGCATCTAGGATGGT	TCGATCATTCTGAAACATCAAAGT	50.0	33.3	57.3	57.0	1707 bp	1.0	40.0