Hotspot

chlL-trnN-GUU

Genome · 108963-109784 · 822 bp

Back to Cyathodium

Pi 0.00281

MarkerSeek score 62.80

Primer available yes

Label chlL-trnN-GUU

Feature Metadata

label_name: chlL-trnN-GUU
feature_type: igs
region: Genome
length_bp: 822
variable_sites: 2
pi: 0.00281
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 62.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlL-trnN-GUU_p1	CCTTTTTCCCACGTCTCGAT	AGGACTTGAACCTACGACCA	50.0	50.0	57.9	58.0	865-975 bp	1.0	83.4
chlL-trnN-GUU_p2	CCACGTCTCGATAGTGCAAT	AGGACTTGAACCTACGACCA	50.0	50.0	57.8	58.0	857-967 bp	1.0	82.5
chlL-trnN-GUU_p3	TCCCTATTCCACCCTTTCCA	AGGACTTGAACCTACGACCA	50.0	50.0	57.6	58.0	811-921 bp	1.0	81.2
chlL-trnN-GUU_p4	AACCTTTTTCCCACGTCTCG	AGGACTTGAACCTACGACCA	50.0	50.0	58.4	58.0	867-977 bp	1.0	80.8
chlL-trnN-GUU_p5	AGCTTGTTGTGGATTTCCCT	AGGACTTGAACCTACGACCA	45.0	50.0	57.3	58.0	826-936 bp	1.0	78.7