Hotspot

trnM-CAU-trnV-GAC

Genome · 79674-80455 · 782 bp

Back to Cyathodium

Pi 0

MarkerSeek score 52.00

Primer available yes

Label trnM-CAU-trnV-GAC

Feature Metadata

label_name: trnM-CAU-trnV-GAC
feature_type: igs
region: Genome
length_bp: 782
variable_sites: 0
pi: 0
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.46
markerseek_score: 52.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnM-CAU-trnV-GAC_p1	TGAACCTGTGAATTCGCCAA	TTTCCAACAACGAGAGAGCC	45.0	50.0	58.0	58.1	534-959 bp	1.0	82.8
trnM-CAU-trnV-GAC_p2	TTGAACCTGTGAATTCGCCA	TTTCCAACAACGAGAGAGCC	45.0	50.0	58.0	58.1	535-960 bp	1.0	82.8
trnM-CAU-trnV-GAC_p3	TAACCGTTCAGCCATGGATG	TTTCCAACAACGAGAGAGCC	50.0	50.0	58.0	58.1	497-922 bp	1.0	82.7
trnM-CAU-trnV-GAC_p4	TTGGGTGCTTTAACCGTTCA	TTTCCAACAACGAGAGAGCC	45.0	50.0	57.9	58.1	507-932 bp	1.0	82.6
trnM-CAU-trnV-GAC_p5	AGTTGGGTGCTTTAACCGTT	TTTCCAACAACGAGAGAGCC	45.0	50.0	57.9	58.1	509-934 bp	1.0	82.5