Hotspot

atpB-rbcL

Genome · 54851-55546 · 696 bp

Back to Cyathodium

Pi 0
MarkerSeek score 51.50
Primer available yes
Label atpB-rbcL

Feature Metadata

label_name
atpB-rbcL
feature_type
igs
region
Genome
length_bp
696
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.42
markerseek_score
51.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpB-rbcL_p1 GGGAGGAGAGGAAACGTCTA GTCTCCGTTTGTGGTGACAT 55.0 50.0 57.9 58.1 415-821 bp 1.0 81.9
atpB-rbcL_p2 ATTAGGCATTTGACCGGGAG GTCTCCGTTTGTGGTGACAT 50.0 50.0 57.7 58.1 430-836 bp 1.0 80.2
atpB-rbcL_p3 GCAACGAGTGTAGACGTACC GTCTCCGTTTGTGGTGACAT 55.0 50.0 58.4 58.1 354-760 bp 1.0 79.9
atpB-rbcL_p4 GGGAGGAGAGGAAACGTCTA CACCTGCTTTAGTCTCCGTT 55.0 50.0 57.9 57.5 426-832 bp 1.0 79.2
atpB-rbcL_p5 GGGAGGAGAGGAAACGTCTA AGCTTTGAATCCAGCACCTG 55.0 50.0 57.9 58.5 440-846 bp 1.0 79.2