Hotspot

rps12-rps7

Genome · 909-965 · 57 bp

Back to Cyathodium

Pi 0.52

MarkerSeek score 75.70

Primer available yes

Label rps12-rps7

Feature Metadata

label_name: rps12-rps7
feature_type: igs
region: Genome
length_bp: 57
variable_sites: 26
pi: 0.52
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.88
markerseek_score: 75.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-rps7_p1	TACGCGTTAGCTACACAACC	ACTTTCTTCCCTCTTCCCGA	50.0	50.0	58.0	58.0	882 bp	0.5	59.6
rps12-rps7_p2	TACGCGTTAGCTACACAACC	TTTTCCTTGTGCCGAGACAA	50.0	45.0	58.0	58.0	750 bp	0.5	59.5
rps12-rps7_p3	TACGCGTTAGCTACACAACC	CGTAAGGGGTTTTGGAGGAG	50.0	55.0	58.0	57.9	1094 bp	0.5	59.0
rps12-rps7_p4	GAGGAGGAAGGGTCAAGGAT	ACTTTCTTCCCTCTTCCCGA	55.0	50.0	58.1	58.0	1175 bp	0.5	58.9
rps12-rps7_p5	GAGGAGGAAGGGTCAAGGAT	TTTTCCTTGTGCCGAGACAA	55.0	45.0	58.1	58.0	1043 bp	0.5	58.7