Hotspot

psaC-ndhE

SSC · 132677-133659 · 983 bp

Back to Cyanotis

Pi 0.132

MarkerSeek score 82.20

Primer available yes

Label psaC-ndhE

Feature Metadata

label_name: psaC-ndhE
feature_type: igs
region: SSC
length_bp: 983
variable_sites: 107
pi: 0.132
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaC-ndhE_p1	CATAAATTTTTACTGAATGTGACA	AAGCAGCCATTGGTTTAGCT	25.0	45.0	52.3	58.1	1097-1178 bp	1.0	51.5
psaC-ndhE_p2	CATAAATTTTTACTGAATGTGACA	GAAGCAGCCATTGGTTTAGC	25.0	50.0	52.3	57.7	1098-1179 bp	1.0	51.5
psaC-ndhE_p3	TCATAAATTTTTACTGAATGTGAC	AAGCAGCCATTGGTTTAGCT	25.0	45.0	52.1	58.1	1098-1179 bp	1.0	51.5
psaC-ndhE_p4	TCATAAATTTTTACTGAATGTGAC	GAAGCAGCCATTGGTTTAGC	25.0	50.0	52.1	57.7	1099-1180 bp	1.0	51.5
psaC-ndhE_p5	CATAAATTTTTACTGAATGTGACA	CGAAGCAGCCATTGGTTTAG	25.0	50.0	52.3	57.5	1099-1180 bp	1.0	51.5