Hotspot

ycf1-ndhF

SSC · 124025-124665 · 641 bp

Back to Cyanotis

Pi 0.316

MarkerSeek score 72.20

Primer available yes

Label ycf1-ndhF

Feature Metadata

label_name: ycf1-ndhF
feature_type: igs
region: SSC
length_bp: 641
variable_sites: 85
pi: 0.316
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.42
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1-ndhF_p1	ATGCCCGGGTAATTTTTGGT	GCGGGCGAATCTCTTCTTAT	45.0	50.0	58.1	57.8	749 bp	0.5	58.3
ycf1-ndhF_p2	TTCGATTACAAGCGCTGGAA	GCGGGCGAATCTCTTCTTAT	45.0	50.0	57.9	57.8	847 bp	0.5	58.0
ycf1-ndhF_p3	TTTCGATTACAAGCGCTGGA	GCGGGCGAATCTCTTCTTAT	45.0	50.0	57.9	57.8	848 bp	0.5	58.0
ycf1-ndhF_p4	CAGAATGCCGTCGCCTATTA	GCGGGCGAATCTCTTCTTAT	50.0	50.0	57.9	57.8	1157 bp	0.5	57.6
ycf1-ndhF_p5	CATGCCCGGGTAATTTTTGG	GCGGGCGAATCTCTTCTTAT	50.0	50.0	57.7	57.8	750 bp	0.5	56.3