Hotspot

rps3-rpl22

LSC · 95641-96414 · 774 bp

Back to Cyanotis

Pi 0.202

MarkerSeek score 83.60

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 774
variable_sites: 150
pi: 0.202
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 83.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	ATGGTGGTGTTGGGTTGTAC	CAAATTGGGGGAATTCGCAC	50.0	50.0	58.0	58.0	1383-1579 bp	1.0	89.6
rps3-rpl22_p2	ATGGTGGTGTTGGGTTGTAC	CACAAATTGGGGGAATTCGC	50.0	50.0	58.0	58.0	1385-1581 bp	1.0	89.6
rps3-rpl22_p3	GCTGGCTGTATAACGTGTCA	CAAATTGGGGGAATTCGCAC	50.0	50.0	58.0	58.0	2020 bp	0.5	59.9
rps3-rpl22_p4	GCTGGCTGTATAACGTGTCA	CACAAATTGGGGGAATTCGC	50.0	50.0	58.0	58.0	2022 bp	0.5	59.9
rps3-rpl22_p5	GCTGGCTGTATAACGTGTCA	TCTGACTTTCGCGAGACATG	50.0	50.0	58.0	58.0	1996 bp	0.5	59.8