Hotspot

rpl33-psaJ

LSC · 85010-85769 · 760 bp

Back to Cyanotis

Pi 0.116

MarkerSeek score 79.30

Primer available yes

Label rpl33-psaJ

Feature Metadata

label_name: rpl33-psaJ
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 71
pi: 0.116
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.80
markerseek_score: 79.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-psaJ_p1	TGAATCGTATGCTTGCGACA	GGCACCAGTAGTAAGCACTC	45.0	55.0	58.0	58.0	984-1045 bp	1.0	90.2
rpl33-psaJ_p2	TGAATCGTATGCTTGCGACA	CATATCTTTCCGTGGCACCA	45.0	50.0	58.0	58.0	997-1058 bp	1.0	89.9
rpl33-psaJ_p3	TGAATCGTATGCTTGCGACA	ACATATCTTTCCGTGGCACC	45.0	50.0	58.0	58.0	998-1059 bp	1.0	89.9
rpl33-psaJ_p4	GCCAGACGTATTGTGTCGAT	GGCACCAGTAGTAAGCACTC	50.0	55.0	58.1	58.0	934-995 bp	1.0	89.8
rpl33-psaJ_p5	TGAATCGTATGCTTGCGACA	ACCCGTGACATTTTGTACCC	45.0	50.0	58.0	58.1	1525-1579 bp	1.0	88.1