Hotspot

accD-psaI

LSC · 68053-69210 · 1158 bp

Back to Cyanotis

Pi 0.151

MarkerSeek score 79.50

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 1158
variable_sites: 125
pi: 0.151
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 79.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	CGTATGCAAGAAGGGAGCTT	AAAGAAGCCATCGCAATTGC	50.0	45.0	58.0	58.0	1411-1616 bp	1.0	90.6
accD-psaI_p2	CGGGCCTAGGTCAACTAAAC	AAAGAAGCCATCGCAATTGC	55.0	45.0	58.0	58.0	1578-1783 bp	1.0	90.1
accD-psaI_p3	GGCCTAGGTCAACTAAACGG	AAAGAAGCCATCGCAATTGC	55.0	45.0	58.0	58.0	1576-1781 bp	1.0	90.1
accD-psaI_p4	GGGCCTAGGTCAACTAAACG	AAAGAAGCCATCGCAATTGC	55.0	45.0	58.0	58.0	1577-1782 bp	1.0	90.1
accD-psaI_p5	GGCCTAGGTCAACTAAACGG	TTGTTTTTCGAGACCCCGTT	55.0	45.0	58.0	58.0	2048-2234 bp	1.0	88.7