Hotspot

ndhC-rbcL

LSC · 59361-61203 · 1843 bp

Back to Cyanotis

Pi 0.137

MarkerSeek score 83.10

Primer available yes

Label ndhC-rbcL

Feature Metadata

label_name: ndhC-rbcL
feature_type: igs
region: LSC
length_bp: 1843
variable_sites: 240
pi: 0.137
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-rbcL_p1	ACCATTCCAACGCTCCTTTT	GCACGACTACTTAACTGGGG	45.0	55.0	58.0	58.0	2835 bp	0.5	59.9
ndhC-rbcL_p2	ACCATTCCAACGCTCCTTTT	ACCGAAATTTTTGGGGACGA	45.0	45.0	58.0	58.0	2462 bp	0.5	59.8
ndhC-rbcL_p3	ACCATTCCAACGCTCCTTTT	TTGAATGCTACTGCGGGTAC	45.0	50.0	58.0	58.0	2915 bp	0.5	59.8
ndhC-rbcL_p4	AACTGGAGAGCTTCTCTGGT	GCACGACTACTTAACTGGGG	50.0	55.0	58.0	58.0	2607 bp	0.5	59.8
ndhC-rbcL_p5	AGCTTCTCTGGTCCTTCACT	GCACGACTACTTAACTGGGG	50.0	55.0	58.0	58.0	2599 bp	0.5	59.8