Hotspot

rps4-trnT-UGU

LSC · 54040-55312 · 1273 bp

Back to Cyanotis

Pi 0.135

MarkerSeek score 80.90

Primer available yes

Label rps4-trnT-UGU

Feature Metadata

label_name: rps4-trnT-UGU
feature_type: igs
region: LSC
length_bp: 1273
variable_sites: 117
pi: 0.135
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.68
markerseek_score: 80.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4-trnT-UGU_p1	CTCCCAGACGGCGTATTTTT	CTCAGAGGTTAGAGCATCGC	50.0	55.0	58.3	57.9	977-1385 bp	1.0	88.7
rps4-trnT-UGU_p2	CTCCCAGACGGCGTATTTTT	GATGGTCATCGGTTCGACTC	50.0	55.0	58.3	58.2	946-1354 bp	1.0	88.6
rps4-trnT-UGU_p3	CTCCCAGACGGCGTATTTTT	ATATGTAGCAAGCAAGCCCG	50.0	50.0	58.3	58.4	1002-1410 bp	1.0	86.4
rps4-trnT-UGU_p4	CTCCCAGACGGCGTATTTTT	CCGCTTAGCTCAGAGGTTAG	50.0	55.0	58.3	57.5	985-1393 bp	1.0	85.7
rps4-trnT-UGU_p5	TAGTCCTGGTAAAGCTCCCA	CTCAGAGGTTAGAGCATCGC	50.0	55.0	57.4	57.9	991-1399 bp	1.0	85.7